FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008167572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008167572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222272
Total Bases38.6 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTG5880.2645407428735963No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG5390.24249568096746327No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA4580.20605384393895765No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC4230.19030737114886265No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT4060.18265908436510223No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC3930.17681039447163835No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC3850.1732112006910452No Hit
GATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAA3360.1511661387849122No Hit
AGATAGAAGTTTGAAGTGGAAAACTGGAAGACAGAAGTACGGGAAGGCGA3230.14531744889144832No Hit
GCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCG3200.14396775122372588No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC3000.13496976677224304No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG2860.128671177656205No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGA2830.1273214799884826No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA2810.1264216815433343No Hit
GTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGATCTTTTAAAAA2540.1142744025338324No Hit
AATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT2500.11247480564353585No Hit
AACCGAAGGTGATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGC2490.1120249064209617No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG2300.10347682119205298No Hit

[OK]Adapter Content

Adapter graph