FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008167617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008167617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27005
Total Bases4.6 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC6952.573597481947788No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC6482.3995556378448435No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC3061.133123495648954No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA2610.9664876874652842No Hit
GTACATGGGCGTCATATCGAAAGATATG2250.8331790409183484No Hit
GTACATGGGCGTCATATCGAAAGATATGACG2020.748009627846695No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1870.6924643584521385No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1800.6665432327346787No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1680.6221070172190335No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC1680.6221070172190335No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA1610.5961858915015739No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT1540.5702647657841141No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT1390.5147194963895575No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA1240.459174226995001No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC1230.45547120903536387No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA1170.43325310127754124No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT1150.425847065358267No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG1040.3851138678022588No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT1010.3740048139233475No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG990.3665987780040733No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG980.3628957600444362No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG940.3480836882058878No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT940.3480836882058878No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT860.31845954452879094No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG860.31845954452879094No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT840.31105350860951675No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG840.31105350860951675No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG830.3073504906498796No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT820.30364747269024256No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC820.30364747269024256No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG820.30364747269024256No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG790.29253841881133125No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA770.28513238289205706No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATG750.2777263469727828No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT750.2777263469727828No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG740.2740233290131457No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA690.2555082392149602No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC680.25180522125532306No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG680.25180522125532306No Hit
GATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACA670.24810220329568597No Hit
CTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCT660.2443991853360489No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG660.2443991853360489No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATG660.2443991853360489No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG640.23699314941677466No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC640.23699314941677466No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC630.23329013145713756No Hit
GCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCT620.2295871134975005No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC620.2295871134975005No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT590.21847805961858913No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG580.21477504165895206No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA570.21107202369931494No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT560.20736900573967784No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG560.20736900573967784No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC560.20736900573967784No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG560.20736900573967784No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC550.20366598778004072No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC550.20366598778004072No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT530.19625995186076653No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT530.19625995186076653No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA530.19625995186076653No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT520.1925569339011294No Hit
GCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCAC500.18515089798185522No Hit
CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA500.18515089798185522No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT500.18515089798185522No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC490.1814478800222181No Hit
GTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGTTAAATATCAA460.1703388261433068No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG460.1703388261433068No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT460.1703388261433068No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC460.1703388261433068No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC460.1703388261433068No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG450.1666358081836697No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA450.1666358081836697No Hit
CTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT450.1666358081836697No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG450.1666358081836697No Hit
CTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAATT450.1666358081836697No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC450.1666358081836697No Hit
GCTCCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAG450.1666358081836697No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG450.1666358081836697No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT440.1629327902240326No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT440.1629327902240326No Hit
ATTAAAGAGTTAGCACCCACCATTGGATGAAGCCCAGGATAAGTGACCCC440.1629327902240326No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC440.1629327902240326No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT440.1629327902240326No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATG440.1629327902240326No Hit
GCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGT430.15922977226439547No Hit
CATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAGCT420.15552675430475837No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC410.15182373634512128No Hit
GTGTATAATGTCTCGGACCCGTCGCAACGGTTGTTAATATGACAGGCCGC410.15182373634512128No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA400.14812071838548418No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT390.14441770042584706No Hit
GGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAAT390.14441770042584706No Hit
GGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCCCTGCATCCA390.14441770042584706No Hit
GGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGAT390.14441770042584706No Hit
ATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG390.14441770042584706No Hit
GTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAG390.14441770042584706No Hit
CATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCA380.14071468246620997No Hit
GGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAG380.14071468246620997No Hit
GAGTACATGGGCGTCATATCGAAAGATATG380.14071468246620997No Hit
CACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGA370.13701166450657284No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA370.13701166450657284No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC360.13330864654693575No Hit
CACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACT360.13330864654693575No Hit
GCCTAAAGGTTCTTGCAGAGCAACATCATATACCCTGTAGAACCCGACTT350.12960562858729863No Hit
TTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTG350.12960562858729863No Hit
CTTCAAATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTT350.12960562858729863No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT350.12960562858729863No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC350.12960562858729863No Hit
GGGTAAGATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATT350.12960562858729863No Hit
GTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCT350.12960562858729863No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA340.12590261062766153No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC340.12590261062766153No Hit
ACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTG330.12219959266802445No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA330.12219959266802445No Hit
AGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTC330.12219959266802445No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA330.12219959266802445No Hit
GCTTGAAGCAGATGATATCCCGATGCTGACGGCTTACAATAAACGTGATC320.11849657470838733No Hit
CATCCACACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTT320.11849657470838733No Hit
CTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCG320.11849657470838733No Hit
GTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGC320.11849657470838733No Hit
GTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATTTCTGATATTTCC320.11849657470838733No Hit
GCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTT320.11849657470838733No Hit
GCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAG320.11849657470838733No Hit
TGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCCTG320.11849657470838733No Hit
GATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGT320.11849657470838733No Hit
GAGTACATGGGCGTCATATCGAAAGATATGACG310.11479355674875025No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC310.11479355674875025No Hit
AATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGA310.11479355674875025No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC310.11479355674875025No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA300.11109053878911312No Hit
ATATGGAATCATTGGAGTGCCAGTAATTCTACCACGCCTTATATTTAAAG300.11109053878911312No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG300.11109053878911312No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT300.11109053878911312No Hit
CCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCC300.11109053878911312No Hit
CCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAATTCTACCACG300.11109053878911312No Hit
GTACATGGGCGTCATATCGAAAGATATGAC290.10738752082947603No Hit
ATATCAGACATTCCTGTAGATAAAATCACCGGCTTGCCTGTTTTTGCCAC290.10738752082947603No Hit
GTTGTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGC290.10738752082947603No Hit
GATGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCC290.10738752082947603No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG280.10368450286983892No Hit
ATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAAA280.10368450286983892No Hit
TGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTG280.10368450286983892No Hit
AATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGT280.10368450286983892No Hit
ATACATAACCTCTGCTGTTGTCCTCCAGAGAGAGATAGAGCGTTTTTATG280.10368450286983892No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)