FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008167664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008167664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19545
Total Bases2.6 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2061.0539779994883602No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1961.002814018930673No Hit
GTACATGGGCGTCATATCGAAAGATATG1830.9363008442056792No Hit
GTACATGGGCGTCATATCGAAAGATATGACG1590.8135072908672295No Hit
TATCAACGCAGAGTACATGGGGGGG1530.782808902532617No Hit
GTATCAACGCAGAGTACATGGGGGGG1400.7162957278076234No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1140.5832693783576363No Hit
GGTATCAACGCAGAGTACATGGGGG1080.5525709900230238No Hit
GTATCAACGCAGAGTACATGGGGAG1060.5423381939114863No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG990.5065234075211051No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC980.5014070094653365No Hit
GTATCAACGCAGAGTACATGGGGGG920.470708621130724No Hit
GTATCAACGCAGAGTACATGGGGTG800.40931184446149915No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG760.3888462522384241No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATG740.3786134561268867No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG670.3427986697365055No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA640.3274494755691993No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG610.31210028140189305No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT600.3069838833461243No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG590.30186748529035556No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT580.2967510872345868No Hit
GTATCAACGCAGAGTACATGGAGAG550.28140189306728064No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG520.26605269889997446No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT520.26605269889997446No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC510.2609363008442057No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATG500.25581990278843697No Hit
GAGTACATGGGCGTCATATCGAAAGATATGACG490.2507035047326682No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA480.24558710667689945No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG460.235354310565362No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA430.22000511639805576No Hit
GAGTACATGGGCGTCATATCGAAAGATATG420.214888718342287No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT410.20977232028651832No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATG400.20465592223074958No Hit
GGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGAT390.19953952417498083No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT380.19442312611921206No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC380.19442312611921206No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC370.18930672806344334No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT360.1841903300076746No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT360.1841903300076746No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT350.17907393195190585No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG340.17395753389613713No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT340.17395753389613713No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA330.1688411358403684No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC330.1688411358403684No Hit
GTATCAACGCAGAGTACATGGGATG330.1688411358403684No Hit
GGTATCAACGCAGAGTACATGGGGGG330.1688411358403684No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT300.15349194167306215No Hit
ATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG300.15349194167306215No Hit
GGTATCAACGCAGAGTACATGGGGGGG280.1432591455615247No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA280.1432591455615247No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA280.1432591455615247No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG280.1432591455615247No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG270.13814274750575595No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC270.13814274750575595No Hit
GGTATCAACGCAGAGTACATGGGGAG270.13814274750575595No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG260.13302634944998723No Hit
GTCTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGG260.13302634944998723No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT250.12790995139421849No Hit
GGTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG250.12790995139421849No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG250.12790995139421849No Hit
CCGTAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGC250.12790995139421849No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC250.12790995139421849No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG240.12279355333844973No Hit
GTATCAACGCAGAGTACATGGGAGG240.12279355333844973No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA240.12279355333844973No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG230.117677155282681No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA230.117677155282681No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT230.117677155282681No Hit
ATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGATTTAATTCTGCATT220.11256075722691225No Hit
ATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGG220.11256075722691225No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC220.11256075722691225No Hit
CCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAG210.1074443591711435No Hit
CACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACT210.1074443591711435No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT210.1074443591711435No Hit
TATCAACGCAGAGTACATGGGGAAG210.1074443591711435No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG210.1074443591711435No Hit
GTACATGGGCGTCATATCGAAAGATATGAC200.10232796111537479No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG200.10232796111537479No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT200.10232796111537479No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG200.10232796111537479No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)