FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008167713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008167713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27416
Total Bases4.5 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC5822.1228479719871607No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC5792.1119054566676394No Hit
GTACATGGGCGTCATATCGAAAGATATGACG2310.8425736796031514No Hit
GTACATGGGCGTCATATCGAAAGATATG2210.80609862853808No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC2190.7988036183250656No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA2000.7295010213014298No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA1840.6711409395973155No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA1640.5981908374671724No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1570.5726583017216225No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT1430.5215932302305223No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1320.4814706740589437No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG1240.45229063320688645No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC1140.41581558214181497No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG1100.4012255617157864No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT1060.3866355412897578No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG1040.3793405310767435No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT980.35745550043770064No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT980.35745550043770064No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT960.3501604902246863No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT940.342865480011672No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA930.33921797490516487No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG900.3282754595856434No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG880.32098044937262915No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC840.3063904289466005No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT790.28815290341406474No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATG780.28450539830755767No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC720.2626203676685147No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG720.2626203676685147No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG710.2589728625620076No Hit
GCTTGAAGCAGATGATATCCCGATGCTGACGGCTTACAATAAACGTGATC700.25532535745550045No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG700.25532535745550045No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG680.24803034724248615No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC680.24803034724248615No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATG670.244382842135979No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATG660.24073533702947186No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC600.21885030639042896No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA560.20426028596440038No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT550.2006127808578932No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC550.2006127808578932No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG540.19696527575138603No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC530.1933177706448789No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA530.1933177706448789No Hit
ATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAAAACTTT520.18967026553837174No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA520.18967026553837174No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT520.18967026553837174No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT520.18967026553837174No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG520.18967026553837174No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG500.18237525532535745No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC500.18237525532535745No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG500.18237525532535745No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG490.17872775021885032No Hit
GAGTACATGGGCGTCATATCGAAAGATATGACG480.17508024511234316No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT480.17508024511234316No Hit
GTATAAGGATGCTCTGTGAACTGTCATCGGTCCGATCAATTAGTCTAGTG470.171432740005836No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC470.171432740005836No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG460.16778523489932887No Hit
GCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGATTTAATTCT460.16778523489932887No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG450.1641377297928217No Hit
CTACTGGGGCGAGTATTCCCAATGTATGGTCAGAATATCCCACAGGGATA450.1641377297928217No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA450.1641377297928217No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG450.1641377297928217No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG440.16049022468631458No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA440.16049022468631458No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA430.15684271957980742No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT430.15684271957980742No Hit
ATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGGATCACATTATG420.15319521447330026No Hit
GCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCG420.15319521447330026No Hit
GCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCT420.15319521447330026No Hit
GCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTT420.15319521447330026No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC420.15319521447330026No Hit
GTCCATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTT410.1495477093667931No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG410.1495477093667931No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG400.14590020426028597No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA400.14590020426028597No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA390.14225269915377883No Hit
GCCCCTGTGGCTGCTGCCACCACAGCTGCTCCTGCTGCTGCTGCAGCCCC390.14225269915377883No Hit
GCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAAC390.14225269915377883No Hit
GATGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCC390.14225269915377883No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT390.14225269915377883No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT390.14225269915377883No Hit
GTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGC380.13860519404727167No Hit
ATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG380.13860519404727167No Hit
CTTCAAATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTT360.13131018383425735No Hit
GAGTACATGGGCGTCATATCGAAAGATATG360.13131018383425735No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC360.13131018383425735No Hit
ACATTGATTGCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGA350.12766267872775022No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA350.12766267872775022No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA350.12766267872775022No Hit
AATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGT350.12766267872775022No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC350.12766267872775022No Hit
GGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAAT340.12401517362124308No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT340.12401517362124308No Hit
CTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAATT340.12401517362124308No Hit
GATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACA340.12401517362124308No Hit
AATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGA340.12401517362124308No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT330.12036766851473593No Hit
GAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATCTTCATAAG330.12036766851473593No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC330.12036766851473593No Hit
GTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCT330.12036766851473593No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG320.11672016340822876No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT310.11307265830172161No Hit
CACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGA310.11307265830172161No Hit
GATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGAC310.11307265830172161No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT310.11307265830172161No Hit
ATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGG310.11307265830172161No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT300.10942515319521448No Hit
GTACATGGGCGTCATATCGAAAGATATGAC300.10942515319521448No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGT300.10942515319521448No Hit
GCCCTAAATAGGATGCAGAGAATATCAAAGCGGAAAAAATCC300.10942515319521448No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT300.10942515319521448No Hit
TCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCCTGATTTT300.10942515319521448No Hit
GTACATGGGGTCATATCGAAAGATATGACG290.10577764808870734No Hit
ATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCA290.10577764808870734No Hit
GCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAG290.10577764808870734No Hit
CGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTC290.10577764808870734No Hit
GTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGTTAAATATCAA280.10213014298220019No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT280.10213014298220019No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)