FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008167786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008167786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32639
Total Bases4.8 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2310.7077422715156714No Hit
GTACATGGGCGTCATATCGAAAGATATGACG1480.45344526486718345No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1440.44118998743834065No Hit
GTACATGGGCGTCATATCGAAAGATATG1350.4136156132234443No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC880.2696161034345415No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA750.22978645179080243No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG750.22978645179080243No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG640.19608443886148472No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG620.18995680014706331No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT620.18995680014706331No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT560.17157388400379914No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA550.16851006464658844No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT520.15931860657495633No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA480.14706332914611353No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA470.14399950978890283No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT470.14399950978890283No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT430.13174423236006005No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA430.13174423236006005No Hit
GAGTACATGGGCGTCATATCGAAAGATATGACG400.12255277428842797No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT390.11948895493121725No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG390.11948895493121725No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC390.11948895493121725No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA390.11948895493121725No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT360.11029749685958516No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG360.11029749685958516No Hit
GAGTACATGGGCGTCATATCGAAAGATATG360.11029749685958516No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC360.11029749685958516No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC350.10723367750237445No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT350.10723367750237445No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTG340.10416985814516377No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG330.10110603878795306No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT330.10110603878795306No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)