FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008167905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008167905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15456
Total Bases2.6 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2751.7792443064182195No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2631.7016045548654244No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1110.718167701863354No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1020.6599378881987578No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG970.6275879917184265No Hit
GTACATGGGCGTCATATCGAAAGATATGACG950.614648033126294No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT940.6081780538302277No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA850.5499482401656315No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA810.5240683229813665No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC660.42701863354037267No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC640.4140786749482402No Hit
GTACATGGGCGTCATATCGAAAGATATG630.4076086956521739No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT600.38819875776397517No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT580.3752587991718426No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA490.3170289855072464No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA480.3105590062111801No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG430.27820910973084884No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG420.2717391304347826No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT410.26526915113871635No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG400.2587991718426501No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA370.23938923395445136No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC370.23938923395445136No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG360.2329192546583851No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT360.2329192546583851No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC360.2329192546583851No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG360.2329192546583851No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG350.22644927536231885No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG350.22644927536231885No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC330.21350931677018634No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT320.2070393374741201No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT320.2070393374741201No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT320.2070393374741201No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT320.2070393374741201No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG310.20056935817805385No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA310.20056935817805385No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC310.20056935817805385No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG300.19409937888198758No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA290.1876293995859213No Hit
CTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAATT280.18115942028985507No Hit
GTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGTTAAATATCAA270.17468944099378883No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT270.17468944099378883No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG270.17468944099378883No Hit
GAGTACATGGGCGTCATATCGAAAGATATGACG260.16821946169772256No Hit
GGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGCTTATGA250.16174948240165632No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG250.16174948240165632No Hit
GGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGAT250.16174948240165632No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA240.15527950310559005No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG240.15527950310559005No Hit
GGGTAAGATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATT240.15527950310559005No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC240.15527950310559005No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC230.1488095238095238No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA230.1488095238095238No Hit
GTTGTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGC230.1488095238095238No Hit
GCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCT230.1488095238095238No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC230.1488095238095238No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATG230.1488095238095238No Hit
CTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTC220.14233954451345754No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA220.14233954451345754No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC220.14233954451345754No Hit
GCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAG220.14233954451345754No Hit
GATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGT220.14233954451345754No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGT210.1358695652173913No Hit
GCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAAC210.1358695652173913No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT210.1358695652173913No Hit
GAGTACATGGGCGTCATATCGAAAGATATG210.1358695652173913No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT210.1358695652173913No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT200.12939958592132506No Hit
CATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCA200.12939958592132506No Hit
CTACTGGGGCGAGTATTCCCAATGTATGGTCAGAATATCCCACAGGGATA200.12939958592132506No Hit
CAATAACATCCGCTCCTAAGGCAACAGAAACTACTGGGGCGAGTATTCCC200.12939958592132506No Hit
GGTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG190.1229296066252588No Hit
GGTCATTGAGCATATCCAAGTGAACAAAGCACCTAAGATGCGCCGCCGGA190.1229296066252588No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG190.1229296066252588No Hit
GATCAGGACCTTCCATATTTTTATCTAAGGTAAAGTGCTTCTCAATAACA190.1229296066252588No Hit
ATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG190.1229296066252588No Hit
GCTATTACCTTCTTATTATTTGATCTAGAAATTGCCCTCCTTTTACCCCT190.1229296066252588No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT190.1229296066252588No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT190.1229296066252588No Hit
CATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCGGCAAT180.11645962732919254No Hit
CACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGA180.11645962732919254No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA180.11645962732919254No Hit
ATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGG180.11645962732919254No Hit
CCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCC180.11645962732919254No Hit
CAAAAAGAAGTGTCTAACAACAACATCCACACTACTACTCCTTGTCATAT170.1099896480331263No Hit
GCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCG170.1099896480331263No Hit
CTCCCTGCCACATTGAGATGATCCTTACGGAAAAGGAACAGATTGTTCCT170.1099896480331263No Hit
CTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT170.1099896480331263No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG170.1099896480331263No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC170.1099896480331263No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG170.1099896480331263No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA170.1099896480331263No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC170.1099896480331263No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATG170.1099896480331263No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT160.10351966873706005No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA160.10351966873706005No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATG160.10351966873706005No Hit
GCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCAC160.10351966873706005No Hit
AATCGAGACGCTGCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTT160.10351966873706005No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT160.10351966873706005No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG160.10351966873706005No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG160.10351966873706005No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)