FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008167952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008167952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54577
Total Bases7 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4050.7420708357000202No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC3990.7310771936896495No Hit
GGTATCAACGCAGAGTACATGGGGG2670.48921706946149474No Hit
GTATCAACGCAGAGTACATGGGGGG2070.3792806493577881No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1710.31331879729556406No Hit
GTATCAACGCAGAGTACATGGGGGGG1680.3078219762903787No Hit
GTACATGGGCGTCATATCGAAAGATATGACG1600.2931637869432179No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1430.26201513458050096No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA1360.24918921890173515No Hit
TATCAACGCAGAGTACATGGGGGGG1330.2436923978965498No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC1290.23636330322296936No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC1230.2253696612125987No Hit
GTATCAACGCAGAGTACATGGGGAG1180.21620829287062318No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT1150.21071147186543784No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA1010.18505964050790627No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1000.18322736683951116No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC980.17956281950272093No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT960.1758982721659307No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA950.1740659984975356No Hit
TATCAACGCAGAGTACATGGGGAATGCG930.17040145116074537No Hit
GTACATGGGCGTCATATCGAAAGATATG900.16490463015556003No Hit
GTATCAACGCAGAGTACATGGGGAATGCG890.16307235648716492No Hit
TATCAACGCAGAGTACATGGGATTG850.15574326181358447No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA820.15024644080839916No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA810.14841416714000405No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG790.14474961980321382No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG780.1429173461348187No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT760.1392527987980285No Hit
GTATCAACGCAGAGTACATGGGATTG740.13558825146123826No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA730.13375597779284315No Hit
GTATCAACGCAGAGTACATGGGGCG730.13375597779284315No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA730.13375597779284315No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT700.1282591567876578No Hit
GTATCAACGCAGAGTACATGGGATG680.12459460945086757No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG670.12276233578247246No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG640.11726551477728712No Hit
GTATCAACGCAGAGTACATGGGGTG620.1136009674404969No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT590.10810414643531159No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG590.10810414643531159No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG590.10810414643531159No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC580.10627187276691648No Hit
GTATCAACGCAGAGTACATGGAGAG580.10627187276691648No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA580.10627187276691648No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT570.10443959909852137No Hit
GTATCAACGCAGAGTACATGGGAGG550.10077505176173114No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)