FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008168029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008168029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243573
Total Bases43.2 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4700.19296063192554183No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC4690.19255007738953003No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA4620.1896761956374475No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT4450.18269676852524705No Hit
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTG3880.15929515997257496No Hit
GCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCG3070.12604024255561985No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG3070.12604024255561985No Hit
GATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAA2840.11659748822734868No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGA2710.11126027925919539No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)