FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008168033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008168033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298716
Total Bases52.9 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA4140.13859317880528663No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT4080.1365845820110071No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG3900.13055879162816858No Hit
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTG3830.12821542870150915No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC3560.1191767431272513No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC3380.11315095274441275No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)