FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008168074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008168074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584777
Total Bases100.7 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCATAGGTTCTCAACCCTCACCCCCACCACGGGAGACTAGAGCTGCAG39900.6823113768154356No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25380.4340115975833523No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT24940.4264873618490467No Hit
GGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACC20810.3558621491611332No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA20750.35483611701554607No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATA19870.33978764554693497No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19800.33859060804375No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT18500.31635991155602905No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17760.3037055150937879No Hit
AGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTC16530.2826718561092519No Hit
CCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCACCACGGGAGA16210.2771996846661206No Hit
GTTCTCAACCCTCACCCCCACCACGGGAGACTAGAGCTGCAGGATCCCAG13740.23496136133945075No Hit
AGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGT12730.21768982022206756No Hit
CTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCACCACGGGAGACTA12450.21290167020932763No Hit
GACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCCTCCCACCCCAAGG12400.21204664342133842No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA11770.20127330589267364No Hit
CCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCT11300.1932360540855745No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT11080.1894739362184217No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC11010.18827689871523673No Hit
GTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCA10910.18656684513925822No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9850.16844027723388574No Hit
CTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAGAAGA9790.1674142450882986No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9610.16433614865153726No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9470.1619420736451673No Hit
TCTCAACCCTCACCCCCACCACGGGAGACTAGAGCTGCAGGATCCCAGGG9430.16125805221477588No Hit
GGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCACCACGGGA9400.16074503614198235No Hit
GGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCT9240.15800895042041668No Hit
GCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAA9010.15407582719566604No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTC8900.15219476826208966No Hit
CCCCAAGGCATCAAGCCCTTCTCCCTGCACTCAATAAACCCTCAATAAAT8740.149458682540524No Hit
TCCCAGGGGAGGGGTCTCTCCTCCCACCCCAAGGCATCAAGCCCTTCTCC8470.14484153788538193No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG8410.14381550573979485No Hit
CACCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCCTCC8380.14330248966700126No Hit
CCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTG8340.14261846823660987No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCG8220.1405664039454356No Hit
GACCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCT8130.1390273557270549No Hit
ATGTCAATGTGTCTGTTGTCATGGCGGAGGTGGACGGCACCTGCTACTGA7680.13133211463515151No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT7670.13116110927755367No Hit
ACCCTCACCCCCACCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGG7540.12893803962878156No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA7500.12825401819839014No Hit
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTG7340.1255179324768245No Hit
CAATAAACCCTCAATAAATATTCTCATTGTCAATCAG7240.12380787890084596No Hit
CCCTCACCCCCACCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGG7230.12363687354324811No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA7190.12295285211285668No Hit
CCCCCACCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCTC7040.1203877717488889No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGC6950.11884872353050821No Hit
GTGTAGAGAACATATATGCATAAACATAGGTCAATTATATGTCTCCATTA6940.11867771817291035No Hit
GAAGACCATCGACCGCTTGGCGGGTAAACCCACCCATGTCAATGTGTCTG6730.11508660566335543No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG6590.11269253065698548No Hit
GCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCC6460.1104694610082134No Hit
ACCTTCTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACA6350.10858840207463699No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAA6240.1067073431410606No Hit
GTGTAAGAAATTGCCATTTGAAACAATCCATTTTGTAACTGAACCTTATG6180.1056813109954735No Hit
GCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTAC6090.1041422627770928No Hit
CCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAGAAGACC6070.1038002520618971No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT5980.10226120384351642No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)