FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008168083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008168083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14472
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2341.616915422885572No Hit
GTACATGGGCGTCATATCGAAAGATATGACG2111.4579878385848535No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1961.3543394140409066No Hit
GTACATGGGCGTCATATCGAAAGATATG1260.8706467661691543No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1120.7739082365948038No Hit
GGTATCAACGCAGAGTACATGGGGG1060.732448866777225No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG850.5873410724156993No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG820.5666113875069099No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG700.4836926478717523No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA680.46987285793255945No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA660.45605306799336653No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG640.44223327805417356No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATG520.35931453841901606No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA500.3454947484798231No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA490.33858485351022666No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC480.33167495854063017No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA480.33167495854063017No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC480.33167495854063017No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT460.31785516860143725No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG440.30403537866224434No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG430.29712548369264785No Hit
GAGTACATGGGCGTCATATCGAAAGATATGACG430.29712548369264785No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT420.29021558872305137No Hit
GTATCAACGCAGAGTACATGGGGGG400.2763957987838585No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATG390.269485903814262No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT370.2556661138750691No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT370.2556661138750691No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT350.24184632393587616No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC320.22111663902708678No Hit
ATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG310.2142067440574903No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG310.2142067440574903No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG290.2003869541182974No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG290.2003869541182974No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGT280.19347705914870095No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG280.19347705914870095No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG270.18656716417910446No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC270.18656716417910446No Hit
GAGTACATGGGCGTCATATCGAAAGATATG270.18656716417910446No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT250.17274737423991154No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT250.17274737423991154No Hit
GAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATCTTCATAAG250.17274737423991154No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT240.16583747927031509No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC240.16583747927031509No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATG240.16583747927031509No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA230.15892758430071863No Hit
GGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCCCTGCATCCA220.15201768933112217No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT220.15201768933112217No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG220.15201768933112217No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC220.15201768933112217No Hit
GTTATAAGACTGACTAGCCCGATGGGCCTCCCAACGGGCCCTCCTCCCCT220.15201768933112217No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT210.14510779436152568No Hit
GGTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG210.14510779436152568No Hit
GCTATTACCTTCTTATTATTTGATCTAGAAATTGCCCTCCTTTTACCCCT210.14510779436152568No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT210.14510779436152568No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA210.14510779436152568No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG210.14510779436152568No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT210.14510779436152568No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA200.13819789939192925No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT200.13819789939192925No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG200.13819789939192925No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG200.13819789939192925No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA190.1312880044223328No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG190.1312880044223328No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC190.1312880044223328No Hit
GTACATGGGGTCATATCGAAAGATATGACG180.12437810945273632No Hit
TTATTGGGGTATCTAACTTTAAGTTTGGAGCTATTTTTGTTTTTAACTTA180.12437810945273632No Hit
TTATAAGACTGACTAGCCCGATGGGCCTCCCAACGGGCCCTCCTCCCCTC170.11746821448313986No Hit
CACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACT170.11746821448313986No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT170.11746821448313986No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG170.11746821448313986No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT160.11055831951354339No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG160.11055831951354339No Hit
CCATAAGACATATGGGCAATCATTATTGGGGTATCTAACTTTAAGTTTGG160.11055831951354339No Hit
GTATCAACGCAGAGTACATGGGGAG150.10364842454394693No Hit
GTACATGGGCGTCATATCGAAAGATATGAC150.10364842454394693No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA150.10364842454394693No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT150.10364842454394693No Hit
GGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGAT150.10364842454394693No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC150.10364842454394693No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT150.10364842454394693No Hit
CCATTGGATGAAGCCCAGGATAAGTGACCCCCCCGGACCTTGGAGTTTCA150.10364842454394693No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA150.10364842454394693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)