FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008168171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008168171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748716
Total Bases300.4 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCC299891.7149154007854908No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA289571.6559006722646787No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA217711.2449705955684056No Hit
CCCCTATTGCGGTCCTCCAGCTCATCTTTCACCTCACCCCCCTCCTCCTC172210.9847796897838185No Hit
CCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTATTGCGGTC169700.9704263013548227No Hit
CTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTAT166470.9519556062848399No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC118290.6764391702254683No Hit
CCTCAGTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTT107460.6145080161672908No Hit
TCTTTCACCTCACCCCCCTCCTCCTCCTTGGCTTTAATTATGCTAATGTT97700.5586956372561354No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC92840.5309038174294739No Hit
CTATTGCGGTCCTCCAGCTCATCTTTCACCTCACCCCCCTCCTCCTCCTT89280.5105460234823722No Hit
GTATAATAGTTATCCCCGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAA86930.49710759208470673No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT86490.49459146024854805No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA77560.4435254209374192No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT73420.4198509077517447No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC69330.39646231863836096No Hit
GTGTACATACTTCCCGGGCGCCCAGCATGGAAATAAAGCACCCAGCGCTG68480.39160160940941807No Hit
GCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTC64540.36907079251290664No Hit
ATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGT62010.3546030344549944No Hit
GGGTAAATGAGTGCGACGGCCGGCAAGCCCCCGCTCCCCGGGCTCTCGCG59620.3409358637994963No Hit
GCCCCCACCTGCTCCTCAGTTCCAGCCTGACCCCCTCCCATCCTTTGGCC59390.33962061306695884No Hit
CCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGAGTGCGAC58760.3360179697560953No Hit
CAACAGGGGAGAGTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTT57340.3278977261030379No Hit
TTTTTCCACAGGGGACCTACCCCTATTGCGGTCCTCCAGCTCATCTTTCA56740.3244666372355488No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG56310.32200769021384834No Hit
CTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATG55860.31943437356323157No Hit
GGACCTACCCCTATTGCGGTCCTCCAGCTCATCTTTCACCTCACCCCCCT54880.3138302617463327No Hit
CCTCCAGCTCATCTTTCACCTCACCCCCCTCCTCCTCCTTGGCTTTAATT51790.29616015407876406No Hit
GGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTAGAG50360.2879827256112485No Hit
GCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAA47690.27271438015092214No Hit
GTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCCCC47520.2717422383051336No Hit
GACCTACCCCTATTGCGGTCCTCCAGCTCATCTTTCACCTCACCCCCCTC46600.2664812353749837No Hit
GAGCTTCAACAGGGGAGAGTGTTAGAGGGAGAAGTGCCCCCACCTGCTCC46510.26596657204486035No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA45210.2585325461653007No Hit
GCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGAC45160.25824662209300997No Hit
TCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT45080.2577891435773447No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG44040.25184192287369705No Hit
GTCACAAAGAGCTTCAACAGGGGAGAGTGTTAGAGGGAGAAGTGCCCCCA43280.24749587697487757No Hit
CTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGA42220.2414342866423136No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACT39660.22679497414102692No Hit
ACCTACCCCTATTGCGGTCCTCCAGCTCATCTTTCACCTCACCCCCCTCC39450.22559409303740574No Hit
CACCTGCTCCTCAGTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGA39450.22559409303740574No Hit
CACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGT39140.22382136378920306No Hit
GGGGAGAGTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGC38930.22262048268558188No Hit
TCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTG38620.22084775343737922No Hit
GACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTAC38480.22004716603496507No Hit
CAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGAGTGCGACGGCCGGCAAG38320.21913220900363467No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA38130.2180456975289298No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC37270.21312780348552882No Hit
ACAGGGGAGAGTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCC35680.2040354179866828No Hit
CAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCT35540.2032348305842687No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG35200.20129054689269157No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA34580.1977450883962862No Hit
GGGGACCTACCCCTATTGCGGTCCTCCAGCTCATCTTTCACCTCACCCCC34550.19757353395291172No Hit
CCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTATTG34500.197287609880621No Hit
GCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTAC34040.19465710841554604No Hit
AGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCCCCTCCCA33880.19374215138421563No Hit
CCCTTTTTCCACAGGGGACCTACCCCTATTGCGGTCCTCCAGCTCATCTT33490.19151194362034774No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGG33370.19082572584684993No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA33070.1891101814131054No Hit
CTCCTCCTCCTTGGCTTTAATTATGCTAATGTTGGAGGAGAATGAATAAA30770.17595767408773064No Hit
GAGTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGAC30570.17481397779856764No Hit
CCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTA30340.17349872706603015No Hit
GGCTTTAATTATGCTAATGTTGGAGGAGAATGAATAAATAAAGTGAATCT30070.17195473707566009No Hit
GACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCT29800.17041074708529003No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC29610.16932423561058513No Hit
CCTCTGACCCTTTTTCCACAGGGGACCTACCCCTATTGCGGTCCTCCAGC29420.16823772413588026No Hit
GAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTAGAGGGAGA29300.16755150636238245No Hit
TCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTATTGCGGT29030.1660075163720124No Hit
AGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGC28980.16572159229972164No Hit
CCCTGTCTCCGGGTAAATGAGTGCGACGGCCGGCAAGCCCCCGCTCCCCG28300.16183302491656737No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC28260.16160428565873475No Hit
CCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTC28120.16080369825632063No Hit
GCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGAG28090.1606321438129462No Hit
CCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCC27580.15771571827558048No Hit
CTGCTCCTCAGTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCC27200.15554269532617074No Hit
GCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGAC26890.15376996607796806No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCC26780.1531409331189284No Hit
GTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGA26480.15142538868518385No Hit
ACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGC25970.14850896314781817No Hit
CATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTG25370.14507787428032912No Hit
CTCCCTGTCTCCGGGTAAATGAGTGCGACGGCCGGCAAGCCCCCGCTCCC25170.1439341779911661No Hit
AGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGA24920.14250455762971231No Hit
GCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTC24460.13987405616463736No Hit
CACGTACCCCGTGTACATACTTCCCGGGCGCCCAGCATGGAAATAAAGCA24460.13987405616463736No Hit
CTTTTTCCACAGGGGACCTACCCCTATTGCGGTCCTCCAGCTCATCTTTC24410.1395881320923466No Hit
ACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGAGTGCGACGGCCGGC24210.1384444358031836No Hit
CCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAG24080.13770103321522764No Hit
CCCCCCTCCTCCTCCTTGGCTTTAATTATGCTAATGTTGGAGGAGAATGA23990.13718636988510427No Hit
CGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTC23480.13426994434773856No Hit
GTCTCCGGGTAAATGAGTGCGACGGCCGGCAAGCCCCCGCTCCCCGGGCT22710.12986671363446095No Hit
GTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGG22470.12849427808746533No Hit
CCTCACCCCCCTCCTCCTCCTTGGCTTTAATTATGCTAATGTTGGAGGAG22160.12672154883926265No Hit
GAAGAGCCTCTCCCTGTCTCCGGGTAAATGAGTGCGACGGCCGGCAAGCC22080.12626407032359743No Hit
GAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCCCCTCCCATCCTT22050.126092515880223No Hit
GCTCCTCAGTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTT21490.12289016627056652No Hit
GTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCA21440.12260424219827576No Hit
GTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCCCCTCCCATCCTTTGG21410.1224326877549013No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG20920.11963063184645191No Hit
GCCTCTCCCTGTCTCCGGGTAAATGAGTGCGACGGCCGGCAAGCCCCCGC20470.11705731519583511No Hit
GAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCA20260.11585643409221394No Hit
GTGTAGAGAACATATATGCATAAACATAGGTCAATTATATGTCTCCATTA20250.1157992492777558No Hit
GAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGG19770.11305437818376456No Hit
CCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGTGGCAGCAG19680.11253971485364118No Hit
GGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTAGA19620.1121966059668923No Hit
CTCTGACCCTTTTTCCACAGGGGACCTACCCCTATTGCGGTCCTCCAGCT19510.11156757300785262No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG19160.10956610450181734No Hit
CAGTATAATAGTTATCCCCGGTGGACGTTCGGCCAAGGGACCAAGGTGGA18990.10859396265602878No Hit
GGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACT18900.10807929932590542No Hit
GTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGGCCC18860.10785056006807281No Hit
CAGGGGACCTACCCCTATTGCGGTCCTCCAGCTCATCTTTCACCTCACCC18750.10722152710903314No Hit
CCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTG18110.10356169898371147No Hit
GAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCC18000.10293266602467181No Hit
AGCTTCAACAGGGGAGAGTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCT17850.10207489380779954No Hit
TGTTGGAGGAGAATGAATAAATAAAGTGAATCTTTGC17810.10184615454996696No Hit
GCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCAC17560.10041653418851318No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)