FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008168456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008168456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933216
Total Bases165.8 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTG18250.19556029900901828No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA15920.1705928745327984No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT13560.1453039810719062No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG10730.11497874018448033No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGA9700.103941638377396No Hit
GCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCG9360.10029832321777594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)