FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008168481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008168481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20003
Total Bases3.3 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC3371.684747287906814No Hit
GTACATGGGCGTCATATCGAAAGATATGACG3201.5997600359946007No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2281.1398290256461532No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1860.9298605209218618No Hit
GTACATGGGCGTCATATCGAAAGATATG1550.7748837674348847No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1430.7148927660850872No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG1360.6798980152977053No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG1190.5949107633854922No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1120.5599160125981103No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA1090.5449182622606609No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT1060.5299205119232115No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG1000.49992501124831273No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA980.48992651102334644No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA940.46992951057341403No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT870.43493475978603213No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG840.4199370094485827No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT820.40993850922361647No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG810.4049392591111333No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT660.32995050742388643No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT660.32995050742388643No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC620.3099535069739539No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATG610.3049542568614708No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT600.2999550067489877No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT590.29495575663650453No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA570.2849572564115383No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG560.27995800629905515No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT560.27995800629905515No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA560.27995800629905515No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT520.25996100584912263No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATG520.25996100584912263No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG520.25996100584912263No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT520.25996100584912263No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC520.25996100584912263No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG520.25996100584912263No Hit
GAGTACATGGGCGTCATATCGAAAGATATGACG510.2549617557366395No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG510.2549617557366395No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG490.24496325551167322No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG490.24496325551167322No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATG480.23996400539919013No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT470.23496475528670702No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA470.23496475528670702No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG460.22996550517422387No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG450.22496625506174076No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA430.2149677548367745No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC430.2149677548367745No Hit
TCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGG420.20996850472429135No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG420.20996850472429135No Hit
GCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCG410.20496925461180823No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG400.1999700044993251No Hit
GCTTACAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGG390.19497075438684197No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG390.19497075438684197No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA380.18997150427435883No Hit
ATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG380.18997150427435883No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA380.18997150427435883No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT360.1799730040493926No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC360.1799730040493926No Hit
TGATTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCC350.17497375393690948No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC350.17497375393690948No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG340.16997450382442633No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC340.16997450382442633No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG340.16997450382442633No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG340.16997450382442633No Hit
GCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGT330.16497525371194321No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT330.16497525371194321No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC320.15997600359946007No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG320.15997600359946007No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGT300.14997750337449384No Hit
AATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGT300.14997750337449384No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC290.1449782532620107No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC290.1449782532620107No Hit
GTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGC280.13997900314952758No Hit
ATACATAAGTGTATAATGTCTCGGACCCGTCGCAACGGTTGTTAATATGA280.13997900314952758No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC280.13997900314952758No Hit
GCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAG280.13997900314952758No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC280.13997900314952758No Hit
GAGTACATGGGCGTCATATCGAAAGATATG280.13997900314952758No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC270.13497975303704443No Hit
GATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGAC270.13497975303704443No Hit
GGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGG270.13497975303704443No Hit
CCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCC270.13497975303704443No Hit
GGTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG260.12998050292456131No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA260.12998050292456131No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA260.12998050292456131No Hit
ATTAAAGAGTTAGCACCCACCATTGGATGAAGCCCAGGATAAGTGACCCC260.12998050292456131No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT250.12498125281207818No Hit
GTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGTTAAATATCAA250.12498125281207818No Hit
GTACATGGGCGTCATATCGAAAGATATGAC250.12498125281207818No Hit
CATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCA250.12498125281207818No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA250.12498125281207818No Hit
GTACATGGGGCTGGTGACGTAGCTGTACGATACAGC250.12498125281207818No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC250.12498125281207818No Hit
GTGTATAATGTCTCGGACCCGTCGCAACGGTTGTTAATATGACAGGCCGC250.12498125281207818No Hit
GAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTT240.11998200269959507No Hit
CCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTAT240.11998200269959507No Hit
AAGGTGTACTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCA240.11998200269959507No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC240.11998200269959507No Hit
GGTTAATAAGGCCTAGCCTAAAGGTTCTTGCAGAGCAACATCATATACCC240.11998200269959507No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT240.11998200269959507No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG240.11998200269959507No Hit
CATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCGGCAAT230.11498275258711194No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT230.11498275258711194No Hit
CTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAATT230.11498275258711194No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC230.11498275258711194No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC230.11498275258711194No Hit
ACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTG220.1099835024746288No Hit
GCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTT220.1099835024746288No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG220.1099835024746288No Hit
GTCAAAACCGCGCAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTG220.1099835024746288No Hit
TCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGAT220.1099835024746288No Hit
ACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAGCTACAAT220.1099835024746288No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG220.1099835024746288No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT210.10498425236214567No Hit
GGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATT210.10498425236214567No Hit
GCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACATGAAAAAACAG210.10498425236214567No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT210.10498425236214567No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT210.10498425236214567No Hit
ATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCA210.10498425236214567No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT210.10498425236214567No Hit
CCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTT210.10498425236214567No Hit
ATGTAGGAGAGAGAGGTTAATAAGGCCTAGCCTAAAGGTTCTTGCAGAGC210.10498425236214567No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)