FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008168527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008168527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42681
Total Bases5.6 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2600.6091703568332514No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2410.564654061526206No Hit
GTACATGGGCGTCATATCGAAAGATATGACG1610.3772170286544364No Hit
GGTATCAACGCAGAGTACATGGGGG1470.34441554790187673No Hit
GTATCAACGCAGAGTACATGGGGGG1410.330357770436494No Hit
GTATCAACGCAGAGTACATGGGGGGG1360.31864295588200836No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1270.29755628968393427No Hit
GTATCAACGCAGAGTACATGGGGAG1130.2647548089313746No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG980.22961036526791778No Hit
TATCAACGCAGAGTACATGGGGGGG950.22258147653522647No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA950.22258147653522647No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA930.21789555071343222No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC750.17572221831728402No Hit
GTACATGGGCGTCATATCGAAAGATATG740.1733792554063869No Hit
GTATCAACGCAGAGTACATGGGGTG720.16869332958459268No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA720.16869332958459268No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG690.16166444085190132No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG640.1499496262974157No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG620.14526370047562145No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT620.14526370047562145No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA600.14057777465382723No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT590.13823481174293012No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG570.13354888592113587No Hit
GAGTACATGGGCGTCATATCGAAAGATATGACG530.1241770342775474No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA520.12183407136665027No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG510.11949110845575314No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT510.11949110845575314No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG500.11714814554485602No Hit
GTATCAACGCAGAGTACATGGGATG490.11480518263395889No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA470.11011925681216467No Hit
GGTATCAACGCAGAGTACATGGGGGG460.10777629390126754No Hit
GTATCAACGCAGAGTACATGGGATTG450.10543333099037042No Hit
GTATCAACGCAGAGTACATGGAGAG430.10074740516857618No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA430.10074740516857618No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA430.10074740516857618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)