FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008168673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008168673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23869
Total Bases4 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC3051.277808035527253No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2120.8881813230550086No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT1260.5278813523817504No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1230.5153127487536135No Hit
GTACATGGGCGTCATATCGAAAGATATGACG1170.49017554149733966No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG950.3980057815576689No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT770.3225941597888475No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA700.29326741798986133No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA680.2848883489044367No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT650.2723197452762998No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA650.2723197452762998No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA610.25556160710545056No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG590.24718253802002596No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA590.24718253802002596No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC580.2429930034773137No Hit
GTACATGGGCGTCATATCGAAAGATATG540.22623486530646444No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT520.21785579622103984No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG490.2052871925929029No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG480.20109765805019064No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT480.20109765805019064No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT470.1969081235074783No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG450.1885290544220537No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT450.1885290544220537No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG440.1843395198793414No Hit
GGTCATTGAGCATATCCAAGTGAACAAAGCACCTAAGATGCGCCGCCGGA390.16339184716577987No Hit
GATCAGGACCTTCCATATTTTTATCTAAGGTAAAGTGCTTCTCAATAACA380.15920231262306758No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC380.15920231262306758No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG360.15082324353764298No Hit
GTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGC360.15082324353764298No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC340.14244417445221835No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG340.14244417445221835No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGT320.13406510536679375No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA320.13406510536679375No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT320.13406510536679375No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC310.12987557082408144No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG310.12987557082408144No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC310.12987557082408144No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG310.12987557082408144No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC300.12568603628136915No Hit
GCTTGAAGCAGATGATATCCCGATGCTGACGGCTTACAATAAACGTGATC290.12149650173865685No Hit
CGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCC290.12149650173865685No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG290.12149650173865685No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT280.11730696719594452No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA280.11730696719594452No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT270.11311743265323222No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG270.11311743265323222No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG270.11311743265323222No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC270.11311743265323222No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT260.10892789811051992No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT260.10892789811051992No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT260.10892789811051992No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC260.10892789811051992No Hit
CTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAATT260.10892789811051992No Hit
CTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCG250.10473836356780762No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG250.10473836356780762No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA250.10473836356780762No Hit
GTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCT250.10473836356780762No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC250.10473836356780762No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC250.10473836356780762No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC240.10054882902509532No Hit
CCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTT240.10054882902509532No Hit
TCATTGGAGTGCCAGTAATTCTACCACGCCTTATATTTAAAGACAAAAAG240.10054882902509532No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG240.10054882902509532No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)