FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008168806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008168806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792603
Total Bases140.6 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA16340.2061561715007387No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT14620.18445552186908198No Hit
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTG12680.15997920774965524No Hit
CCCTGGGAGACAGTGATAATGAAAGCTAAGCCTCGGGCTAATTTCCCCAT10160.12818523270792567No Hit
GCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCG9630.12149840462375237No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG9360.11809190729785277No Hit
AATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT8980.11329757772806814No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGA8510.10736774904965034No Hit
GATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAA8420.10623224994101713No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)