FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008168838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008168838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506115
Total Bases89.4 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA8610.17011943925787618No Hit
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTG7770.15352242079369313No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT7680.1517441688153878No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG5770.1140057101646859No Hit
GTCTAGAGCTTGTCTCAATGGATCTAGAACTTCATCGCCCTCTGATCGCC5510.10886853778291496No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG5430.10728786935775465No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)