FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008169131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008169131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486622
Total Bases81.7 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG6240.12823094722392328No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA5880.12083300796100466No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA5820.11960001808385154No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC5610.11528455351381564No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA5330.1095306007537678No Hit
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTG5320.10932510244090896No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC4900.1006941733008372No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA4880.10028317667511949No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)