FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008169226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008169226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726211
Total Bases126.5 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC95401.3136677907660446No Hit
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG81821.1266697970699977No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT71320.9820837194699611No Hit
GTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTG50320.6929115642698885No Hit
GGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCC45150.6217201336801563No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC41010.5647119087978563No Hit
CTACTAAACTGGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCC40040.5513549092481387No Hit
GAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCT38740.5334537758309913No Hit
GCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTA37950.5225753947544172No Hit
GGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGC37710.5192705701235591No Hit
GTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGC37450.5156903434401297No Hit
CTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGA36480.5023333438904121No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA36060.4965499007864106No Hit
GAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGT33630.4630885513989736No Hit
CACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCC33180.45689200521611484No Hit
GTGCCTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTT30700.42274215069724913No Hit
GTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGC30350.41792261477724796No Hit
GCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCA30350.41792261477724796No Hit
GAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGC30110.41461779014638994No Hit
GTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTT28030.38597597667895417No Hit
GTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCG26160.36022588476351913No Hit
TCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATA25600.3525146272915172No Hit
GGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAA24880.3426001533989433No Hit
CTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCA24170.3328233805326551No Hit
TCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAG24000.3304824630857974No Hit
GTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCT23860.3285546487177969No Hit
ATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCAC23780.3274530405075109No Hit
CTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTG23600.3249744220343674No Hit
CACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTA23350.3215318963772237No Hit
CACCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTG22580.31092891735322103No Hit
CCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGC22280.30679788656464857No Hit
GGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGC21680.29853582498750364No Hit
CCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCT21650.29812272190864636No Hit
ACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTG21350.29399169112007395No Hit
CTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACTGGGGGATA20300.27953308336007027No Hit
GTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTAGCCGTTCCTCC20260.27898227925492725No Hit
CTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAA20000.27540205257149786No Hit
GCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAA19990.27526435154521206No Hit
CCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACC19380.2668645889417814No Hit
CTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTA18750.2581894242857792No Hit
GGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATC18500.2547468986286355No Hit
CCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTG18050.24855035244577675No Hit
CCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAAT17300.23822277547434564No Hit
CCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATG17110.2356064559749164No Hit
GTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGAC16530.22761979645034297No Hit
CCGTTAAGCTGGAGCCTCGGTAGCCGTTCCTCCTGCCCGCTGGGCCTCCC16010.220459343083484No Hit
GCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCT15870.21853152871548354No Hit
GGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGG15180.20903015790176685No Hit
GTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAAC14630.20145660145605065No Hit
CAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTC14240.19608626143090643No Hit
CCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCT14130.1945715501417632No Hit
ATATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTT13670.18823730293261876No Hit
CTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCCA13560.18672259164347552No Hit
CCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAAT13490.18575868445947527No Hit
GCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCA13440.18507017932804654No Hit
CCCTAAGTCCAACTACTAAACTGGGGGATATTATGAAGGGCCTTGAGCAT13250.1824538598286173No Hit
TCCTTGCACCGGCCCTTCCTGGTCTTTGAATAAAGTCTGAGTGGGCGGC13090.1802506434080453No Hit
CCTCCAAATACCGTTAAGCTGGAGCCTCGGTAGCCGTTCCTCCTGCCCGC12410.1708869736206144No Hit
ATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTAT12320.16964766438404266No Hit
GCCCTGGGCAGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGA11700.16111020075432622No Hit
CACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTC11620.16000859254404023No Hit
GCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTATTTTCATTGC11520.15863158228118274No Hit
CCTTTGTTCCCTAAGTCCAACTACTAAACTGGGGGATATTATGAAGGGCC10980.15119572686175228No Hit
GTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTG10950.15078262378289506No Hit
GTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCCACACTG10750.14802860325718006No Hit
AGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGG10670.14692699504689408No Hit
GCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCA10520.14486147965260787No Hit
GTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGA10250.14114355194289263No Hit
TTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCC10230.14086814989032112No Hit
GAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGT9790.1348093047337482No Hit
GCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGACAAC9640.13274378933946196No Hit
CCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCC9440.12998976881374696No Hit
CACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTT9260.1275111503406035No Hit
TCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGT9200.126684944182889No Hit
CCTTTGGGGATCTGTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTG9190.12654724315660323No Hit
TCCAAATACCGTTAAGCTGGAGCCTCGGTAGCCGTTCCTCCTGCCCGCTG9110.12544563494631725No Hit
CCTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCA8820.12145230518403054No Hit
TGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGACA8710.1199375938948873No Hit
CCTCCCCTCCTTGCACCGGCCCTTCCTGGTCTTTGAATAAAGTCTGAGTG8680.11952449081603005No Hit
TCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGT8650.11911138773717282No Hit
GGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCA8290.11415415079088584No Hit
CGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGC8240.11346564565945709No Hit
GGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGA8210.11305254258059987No Hit
GGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGG8140.1120886353965996No Hit
TCTGGATTCTGCCTAATAAAAAACATTTATTTTCATTGC7980.10988541897602763No Hit
GGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACA7890.10864610973945589No Hit
GATCCTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGC7720.10630519229259815No Hit
GGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGC7400.10189875945145419No Hit
TGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTAG7370.10148565637259695No Hit
CTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAA7360.1013479553463112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)