FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008169385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008169385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300852
Total Bases51.1 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG8370.2782098839296398No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG6220.20674617419860927No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC5510.1831465305199899No Hit
GTCTAGAGCTTGTCTCAATGGATCTAGAACTTCATCGCCCTCTGATCGCC5280.17550157552550757No Hit
CTATAAAGGTCAGCAAATTGGCAAAGTAGTCCAGGTTTACAGGAAGAAAT4700.15622299336550863No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA4700.15622299336550863No Hit
TATTTATGCTGTCTGAAGGTCACGATCATGTTACCATATCAAGCTGAAAA4650.1545610466275777No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA4630.1538962679324053No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC4220.14026830468137158No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA4080.13561485381516492No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4050.13461768577240635No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC3930.1306290136013721No Hit
CTATATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATACAT3730.12398122664964834No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA3730.12398122664964834No Hit
GCTATATACTTTGGTTACCTATGTACCTGTTACCACTTTCAAAAATCTAC3590.1193277757834417No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC3360.11168282078895936No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG3320.1103532633986146No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT3230.1073617592703389No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC3190.10603220187999415No Hit
GCATAATATTTCGCCCACTAAGCCAATCACTTTATTGACTCCTAGCCGCA3120.10370547644689082No Hit
CATTAAAGCAGCCGGTGTAAATGTTGAGCCTTTTTGGCCTGGCTTGTTTG3110.10337308709930464No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)