FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008169582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008169582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences615582
Total Bases107.3 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTG19190.3117375101936054No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA15400.25016975805010544No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT13480.21897976224126114No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG11030.17918002800601707No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG10900.17706820537312656No Hit
GATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAA10170.16520950904997223No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGA10000.1624478948377308No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA9810.1593613848358139No Hit
GCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCG9780.15887404115130072No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC9760.15854914536162526No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA8920.14490352219525587No Hit
AGATAGAAGTTTGAAGTGGAAAACTGGAAGACAGAAGTACGGGAAGGCGA8340.1354815442946675No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG8150.1323950342927506No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT7780.12638446218375454No Hit
AACCGAAGGTGATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGC7570.12297305639216222No Hit
AATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT7350.11939920270573212No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT7020.11403842217608702No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA6900.11208904743803426No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA6840.11111436006900786No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGA6610.10737805848774006No Hit
GTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGATCTTTTAAAAA6580.10689071480322687No Hit
AGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAG6350.10315441322195906No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA6270.10185483006325723No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG6190.10055524690455535No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)