FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008169619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008169619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29369
Total Bases3.9 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2560.8716674044060063No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2510.8546426504137015No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC1940.6605604549014267No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA1820.6197010453198951No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC1310.44604855459838605No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1300.4426436037999251No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA1150.3915693418230106No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA1020.34730498144301813No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1010.3439000306445572No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA990.33709012904763525No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT980.33368517824917426No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA970.33028022745071334No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG860.29282576866764276No Hit
GTATCAACGCAGAGTACATGGGGGGG800.272396063876877No Hit
TATCAACGCAGAGTACATGGGGGGG780.265586162279955No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA770.2621812114814941No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT760.2587762606830331No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG760.2587762606830331No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA750.25537130988457213No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT730.24856140828765022No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG720.24515645748918927No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT710.24175150669072834No Hit
GTATCAACGCAGAGTACATGGGGTG680.23153665429534545No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG650.22132180189996256No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA620.21110694950457967No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA620.21110694950457967No Hit
GTATCAACGCAGAGTACATGGGATG610.2077019987061187No Hit
CATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAA610.2077019987061187No Hit
GTATCAACGCAGAGTACATGGGGGG610.2077019987061187No Hit
ACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAA610.2077019987061187No Hit
GTATCAACGCAGAGTACATGGGGAG600.20429704790765774No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC590.20089209710919675No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT590.20089209710919675No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC580.1974871463107358No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC570.19408219551227485No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT550.18727229391535294No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT550.18727229391535294No Hit
GCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAAC550.18727229391535294No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCC540.18386734311689196No Hit
GATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCA530.180462392318431No Hit
GGTATCAACGCAGAGTACATGGGGG510.17365249072150907No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT490.16684258912458713No Hit
GTATCAACGCAGAGTACATGGGAGG480.16343763832612618No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG470.16003268752766522No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG450.15322278593074332No Hit
GCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCG440.14981783513228233No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA430.14641288433382138No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT430.14641288433382138No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA430.14641288433382138No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA410.13960298273689944No Hit
GTTATAAGACTGACTAGCCCGATGGGCCTCCCAACGGGCCCTCCTCCCCT400.1361980319384385No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG390.1327930811399775No Hit
GCTCCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAG390.1327930811399775No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT380.12938813034151656No Hit
AGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATT380.12938813034151656No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT370.1259831795430556No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG370.1259831795430556No Hit
CCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCC370.1259831795430556No Hit
CGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCC360.12257822874459463No Hit
TGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGC360.12257822874459463No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC350.1191732779461337No Hit
CATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCGGCAAT350.1191732779461337No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA350.1191732779461337No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC350.1191732779461337No Hit
TGATTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCC340.11576832714767273No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC340.11576832714767273No Hit
GTCTTAAGTTAAGCAACTCTGCGTTGATACCACTGCTTCCGCGGACAGGC340.11576832714767273Clontech Universal Primer Mix Long (96% over 25bp)
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG340.11576832714767273No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT340.11576832714767273No Hit
CTCGTAGACGGCATCTCACAGTCACTGAAGGCTATTAAAGAGTTAGCACC340.11576832714767273No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA340.11576832714767273No Hit
GCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGATTTAATTCT330.11236337634921176No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA320.10895842555075079No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC310.10555347475228984No Hit
TATCAACGCAGAGTACATGGGGTTTTGT310.10555347475228984No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC310.10555347475228984No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG310.10555347475228984No Hit
ACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACC300.10214852395382887No Hit
TAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGAT300.10214852395382887No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA300.10214852395382887No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG300.10214852395382887No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)