FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008169623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008169623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274056
Total Bases47.6 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG4900.1787955746270835No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC4510.16456490644247893No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC4420.16128090609218554No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG4310.15726712788627142No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4310.15726712788627142No Hit
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTG4190.15288846075254692No Hit
GCATAATATTTCGCCCACTAAGCCAATCACTTTATTGACTCCTAGCCGCA4150.1514289050413054No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA4020.14668534897977056No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT3500.12771112473363108No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA3240.11822401261056134No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA3160.11530490118807835No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC2860.10435823335376712No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)