FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008169817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008169817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11917
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2912.4418897373500044No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2261.896450448938491No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1211.015356213812201No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT880.7384408827725099No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA810.6797012670974238No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT780.6545271460938156No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA760.6377443987580768No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT690.5790047830829907No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA680.5706134094151213No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT680.5706134094151213No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG670.5622220357472518No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG670.5622220357472518No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG670.5622220357472518No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG670.5622220357472518No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA670.5622220357472518No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA620.5202651674079047No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT600.5034824200721658No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG580.48669967273642695No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT530.4447428043970798No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA520.4363514307292104No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT500.41956868339347153No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG500.41956868339347153No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT480.40278593605773266No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG470.39439456238986326No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT450.3776118150541244No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT450.3776118150541244No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC450.3776118150541244No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA400.33565494671477725No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG400.33565494671477725No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC380.3188721993790384No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC370.3104808257111689No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT360.3020894520432995No Hit
GTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGC360.3020894520432995No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT350.29369807837543005No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG340.28530670470756064No Hit
GCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCG330.2769153310396912No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG330.2769153310396912No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG320.2685239573718218No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC310.26013258370395237No Hit
TGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTG300.2517412100360829No Hit
GGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATT300.2517412100360829No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT290.24334983636821347No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC290.24334983636821347No Hit
GAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTT280.23495846270034404No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG280.23495846270034404No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC270.22656708903247463No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA270.22656708903247463No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT270.22656708903247463No Hit
TGATTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCC260.2181757153646052No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG260.2181757153646052No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC260.2181757153646052No Hit
GGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGCTTATGA250.20978434169673577No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG250.20978434169673577No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG240.20139296802886633No Hit
CTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCG240.20139296802886633No Hit
AGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCA240.20139296802886633No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT240.20139296802886633No Hit
GTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGTTAAATATCAA230.1930015943609969No Hit
TCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGG230.1930015943609969No Hit
CACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGA230.1930015943609969No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT230.1930015943609969No Hit
AGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTA230.1930015943609969No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC230.1930015943609969No Hit
GCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGATTTAATTCT230.1930015943609969No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT220.18461022069312746No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA220.18461022069312746No Hit
GTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATTTCTGATATTTCC220.18461022069312746No Hit
CAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTT220.18461022069312746No Hit
GCTTGAAGCAGATGATATCCCGATGCTGACGGCTTACAATAAACGTGATC210.17621884702525803No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC210.17621884702525803No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC210.17621884702525803No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG210.17621884702525803No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC210.17621884702525803No Hit
CTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT210.17621884702525803No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC210.17621884702525803No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG210.17621884702525803No Hit
GCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTT200.16782747335738862No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG200.16782747335738862No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC200.16782747335738862No Hit
ATATCAGACATTCCTGTAGATAAAATCACCGGCTTGCCTGTTTTTGCCAC200.16782747335738862No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC200.16782747335738862No Hit
CATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCA200.16782747335738862No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG200.16782747335738862No Hit
CCACACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATT190.1594360996895192No Hit
CCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCC190.1594360996895192No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC190.1594360996895192No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA190.1594360996895192No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT180.15104472602164976No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT180.15104472602164976No Hit
GCCTAAAGGTTCTTGCAGAGCAACATCATATACCCTGTAGAACCCGACTT180.15104472602164976No Hit
CCATTGGATGAAGCCCAGGATAAGTGACCCCCCCGGACCTTGGAGTTTCA180.15104472602164976No Hit
ATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAAA180.15104472602164976No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC180.15104472602164976No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC180.15104472602164976No Hit
TCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCCTGATTTT180.15104472602164976No Hit
CTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAATT180.15104472602164976No Hit
ACATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAA180.15104472602164976No Hit
GGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTG180.15104472602164976No Hit
GCTCCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAG180.15104472602164976No Hit
GTACTGGTAGGCGTTAGACCCCGTGACTCGTGGCTGAACGGCCGTACAAC180.15104472602164976No Hit
GGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAAT170.14265335235378032No Hit
GATCATGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCAT170.14265335235378032No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA170.14265335235378032No Hit
CCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTT170.14265335235378032No Hit
ATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTAT170.14265335235378032No Hit
TTCCTATTGTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAAT170.14265335235378032No Hit
GCCTTATATTTAAAGACAAAAAGAAGTGTCTAACAACAACATCCACACTA170.14265335235378032No Hit
TCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAGC170.14265335235378032No Hit
AGTTTACTGCGTCACGCTGAGGGACTCATCCTGGGCTACAATCCTATTGC170.14265335235378032No Hit
CTCCTAAGGCAACAGAAACTACTGGGGCGAGTATTCCCAATGTATGGTCA170.14265335235378032No Hit
GCTTACAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGG160.1342619786859109No Hit
CATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAGCT160.1342619786859109No Hit
CATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCGGCAAT160.1342619786859109No Hit
CTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTC160.1342619786859109No Hit
GAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATCTTCATAAG160.1342619786859109No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT160.1342619786859109No Hit
GCCCTAAATAGGATGCAGAGAATATCAAAGCGGAAAAAATCC160.1342619786859109No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG160.1342619786859109No Hit
CTACTGGGGCGAGTATTCCCAATGTATGGTCAGAATATCCCACAGGGATA160.1342619786859109No Hit
GCTTGAGGAGCTTGAAGCAGATGATATCCCGATGCTGACGGCTTACAATA150.12587060501804145No Hit
ATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAA150.12587060501804145No Hit
GTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCG150.12587060501804145No Hit
GCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACATGAAAAAACAG150.12587060501804145No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG150.12587060501804145No Hit
GAATCATTGGAGTGCCAGTAATTCTACCACGCCTTATATTTAAAGACAAA150.12587060501804145No Hit
GAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATAT150.12587060501804145No Hit
CATCCACACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTT150.12587060501804145No Hit
GTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCT150.12587060501804145No Hit
GGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCTGAGGGACTCA150.12587060501804145No Hit
GATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGAC150.12587060501804145No Hit
GCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGT150.12587060501804145No Hit
ATTAAAGAGTTAGCACCCACCATTGGATGAAGCCCAGGATAAGTGACCCC140.11747923135017202No Hit
ATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCC140.11747923135017202No Hit
TTCTTATTGGATTTCTGATATTTCCTATTGTTATGGGCTATTACATCTCT140.11747923135017202No Hit
GCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAG140.11747923135017202No Hit
TTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGA140.11747923135017202No Hit
ATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGGATCACATTATG140.11747923135017202No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC140.11747923135017202No Hit
CTCGTAGACGGCATCTCACAGTCACTGAAGGCTATTAAAGAGTTAGCACC140.11747923135017202No Hit
ATGTAGGAGAGAGAGGTTAATAAGGCCTAGCCTAAAGGTTCTTGCAGAGC140.11747923135017202No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC140.11747923135017202No Hit
GGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG130.1090878576823026No Hit
GATGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCC130.1090878576823026No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT130.1090878576823026No Hit
ATAATATCCCTGCATCCATTATTTTCTAAAACTTTAACTGCTTCCCAAAT130.1090878576823026No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA130.1090878576823026No Hit
TGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGC130.1090878576823026No Hit
GCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTT130.1090878576823026No Hit
GATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGT130.1090878576823026No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA130.1090878576823026No Hit
GCGTTAGACCCCGTGACTCGTGGCTGAACGGCCGTACAACTCGACAGCCG130.1090878576823026No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC130.1090878576823026No Hit
GTCTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGG130.1090878576823026No Hit
TAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATC120.10069648401443317No Hit
CTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAA120.10069648401443317No Hit
ATATGAGATAATATCCTTATCTTGAGACGCATAGCAAATGTAGGAGAGAG120.10069648401443317No Hit
GCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAAC120.10069648401443317No Hit
AGCACGATCGGCTCCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGG120.10069648401443317No Hit
GTCCATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTT120.10069648401443317No Hit
GTGTTGTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGT120.10069648401443317No Hit
ATGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCCT120.10069648401443317No Hit
CACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACT120.10069648401443317No Hit
GATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACA120.10069648401443317No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA120.10069648401443317No Hit
ATCCCGGAAGATACGCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGAT120.10069648401443317No Hit
GTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTC120.10069648401443317No Hit
CCTCCATATATCCAAACAACAAAGCATAATATTTCGCCCACTAAGCCAAT120.10069648401443317No Hit
CATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCA120.10069648401443317No Hit
GTGTATAATGTCTCGGACCCGTCGCAACGGTTGTTAATATGACAGGCCGC120.10069648401443317No Hit
GCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGC120.10069648401443317No Hit
TAACGATAGCGACTCTGTCTCAGGGGATCTGCATATGTTTGCAGCATACT120.10069648401443317No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)