FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008169921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008169921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13689
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC3502.5567974285923003No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2681.9577763167506756No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1881.3733654759295786No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT1030.7524289575571627No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA920.6720724669442618No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA920.6720724669442618No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG860.6282416538826796No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT750.5478851632697787No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA740.540580027759515No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC740.540580027759515No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA720.5259697567389875No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT690.5040543502081963No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG680.49674921469793265No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT630.46022353714661407No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG620.4529184016363504No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT600.43830813061582297No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG600.43830813061582297No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT590.4310029951055592No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA560.40908758857476807No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT500.3652567755131858No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG500.3652567755131858No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG500.3652567755131858No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT490.35795164000292207No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT490.35795164000292207No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC480.35064650449265833No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT480.35064650449265833No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG430.3141208269413398No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT420.30681569143107607No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA420.30681569143107607No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG400.29220542041054864No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG380.2775951493900212No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT370.2702900138797575No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC370.2702900138797575No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC370.2702900138797575No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC360.26298487836949375No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG360.26298487836949375No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG360.26298487836949375No Hit
GGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGAT350.25567974285923006No Hit
CTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCT350.25567974285923006No Hit
CCATTGGATGAAGCCCAGGATAAGTGACCCCCCCGGACCTTGGAGTTTCA340.24837460734896633No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG340.24837460734896633No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG340.24837460734896633No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG340.24837460734896633No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC330.24106947183870261No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC330.24106947183870261No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA320.2337643363284389No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT320.2337643363284389No Hit
GCCTAAAGGTTCTTGCAGAGCAACATCATATACCCTGTAGAACCCGACTT310.2264592008181752No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG310.2264592008181752No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT300.21915406530791148No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT300.21915406530791148No Hit
CACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGA300.21915406530791148No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT300.21915406530791148No Hit
CACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACT290.21184892979764774No Hit
CTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT290.21184892979764774No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA290.21184892979764774No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT280.20454379428738403No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA280.20454379428738403No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC280.20454379428738403No Hit
GCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGT270.19723865877712032No Hit
CATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCGGCAAT270.19723865877712032No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA270.19723865877712032No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC270.19723865877712032No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC270.19723865877712032No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA270.19723865877712032No Hit
GCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGATTTAATTCT270.19723865877712032No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG260.1899335232668566No Hit
GTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAG260.1899335232668566No Hit
GCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTT250.1826283877565929No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA250.1826283877565929No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG250.1826283877565929No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG240.17532325224632916No Hit
ATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAA240.17532325224632916No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA240.17532325224632916No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC240.17532325224632916No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA240.17532325224632916No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG230.16801811673606545No Hit
GCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCG230.16801811673606545No Hit
GAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTT220.16071298122580174No Hit
GCTTGAAGCAGATGATATCCCGATGCTGACGGCTTACAATAAACGTGATC220.16071298122580174No Hit
CTATTAAAGAGTTAGCACCCACCATTGGATGAAGCCCAGGATAAGTGACC220.16071298122580174No Hit
GATGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCC220.16071298122580174No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT220.16071298122580174No Hit
GGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGCTTATGA210.15340784571553803No Hit
GCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAAC210.15340784571553803No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT210.15340784571553803No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT210.15340784571553803No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC200.14610271020527432No Hit
GGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAAT190.1387975746950106No Hit
ATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAAA190.1387975746950106No Hit
CAATAAAATAATATCCCTGCATCCATTATTTTCTAAAACTTTAACTGCTT190.1387975746950106No Hit
AATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGT190.1387975746950106No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC190.1387975746950106No Hit
ATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGGATCACATTATG190.1387975746950106No Hit
GCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAATTCTACC180.13149243918474687No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA180.13149243918474687No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG180.13149243918474687No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA180.13149243918474687No Hit
GTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCT180.13149243918474687No Hit
CATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCA180.13149243918474687No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC170.12418730367448316No Hit
CATCCACACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTT170.12418730367448316No Hit
TGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGC170.12418730367448316No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG170.12418730367448316No Hit
CTCGTAGACGGCATCTCACAGTCACTGAAGGCTATTAAAGAGTTAGCACC170.12418730367448316No Hit
CTTCAAATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTT170.12418730367448316No Hit
GTGTATAATGTCTCGGACCCGTCGCAACGGTTGTTAATATGACAGGCCGC170.12418730367448316No Hit
AGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTA170.12418730367448316No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC170.12418730367448316No Hit
TTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCC160.11688216816421945No Hit
GGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCCCTGCATCCA160.11688216816421945No Hit
CTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTC160.11688216816421945No Hit
TCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGCCG160.11688216816421945No Hit
GCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAG160.11688216816421945No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA160.11688216816421945No Hit
GCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCT160.11688216816421945No Hit
GTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGTTAAATATCAA150.10957703265395574No Hit
CATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAGCT150.10957703265395574No Hit
GGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATT150.10957703265395574No Hit
CATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTG150.10957703265395574No Hit
ACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAGCTACAAT150.10957703265395574No Hit
GGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG150.10957703265395574No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC150.10957703265395574No Hit
ATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGG150.10957703265395574No Hit
GTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGC150.10957703265395574No Hit
GTATGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTTTTTGCGA150.10957703265395574No Hit
GATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGT150.10957703265395574No Hit
GCGTTAGACCCCGTGACTCGTGGCTGAACGGCCGTACAACTCGACAGCCG150.10957703265395574No Hit
GTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATTTCTGATATTTCC150.10957703265395574No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT150.10957703265395574No Hit
TGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTG140.10227189714369202No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC140.10227189714369202No Hit
ACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGATTTGAG140.10227189714369202No Hit
ATACATAAGTGTATAATGTCTCGGACCCGTCGCAACGGTTGTTAATATGA140.10227189714369202No Hit
GTACATGGGGGATCAAGCGACTAAGTGCATATGGTGGATGCCTTGGCGAT140.10227189714369202No Hit
CCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCC140.10227189714369202No Hit
AGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCA140.10227189714369202No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC140.10227189714369202No Hit
GCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCCCCGTCCTGAT140.10227189714369202No Hit
GCTCCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAG140.10227189714369202No Hit
GATTGCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGATTTAA140.10227189714369202No Hit
GAGTTAGCACCCACCATTGGATGAAGCCCAGGATAAGTGACCCCCCCGGA140.10227189714369202No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC140.10227189714369202No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)