FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008170094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008170094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813103
Total Bases136.7 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG24960.3069721794163839No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA18330.22543269425890694No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC17460.21473294281290317No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA15750.19370239686730956No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA14940.18374055931413363No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT11930.14672187902393669No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG10310.12679820391758484No Hit
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC10230.12581431872714774No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC9780.1202799645309389No Hit
GCATAATATTTCGCCCACTAAGCCAATCACTTTATTGACTCCTAGCCGCA9470.11646740941799502No Hit
CTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCAC9250.1137617251442929No Hit
CCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGC8260.10158614591263344No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)