FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008170113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008170113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11142
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC3022.7104649075569918No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2552.2886375875067313No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1171.050080775444265No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA950.8526296894632921No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG880.7898043439238916No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT870.78082929456112No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC860.7718542451983487No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA840.7539041464728056No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT690.6192784060312331No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG680.6103033566684617No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT670.6013283073056902No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT650.5833782085801472No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT650.5833782085801472No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA620.5564530604918327No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA600.5385029617662896No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG560.5026027643152038No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG560.5026027643152038No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG530.47567761622688925No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT520.4667025668641177No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT480.4308023694130318No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG480.4308023694130318No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC480.4308023694130318No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT480.4308023694130318No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA450.40387722132471726No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA410.3679770238736313No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT370.3320768264225453No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC370.3320768264225453No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT370.3320768264225453No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT370.3320768264225453No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT350.31412672769700234No Hit
GCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCG350.31412672769700234No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT340.30515167833423085No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC340.30515167833423085No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG330.2961766289714593No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT320.28720157960868786No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC310.27822653024591637No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC310.27822653024591637No Hit
GCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTT300.2692514808831448No Hit
GCTTGAAGCAGATGATATCCCGATGCTGACGGCTTACAATAAACGTGATC290.2602764315203734No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG290.2602764315203734No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG290.2602764315203734No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG290.2602764315203734No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT280.2513013821576019No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG280.2513013821576019No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC280.2513013821576019No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT270.24232633279483037No Hit
GGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGAT270.24232633279483037No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG270.24232633279483037No Hit
GCTCCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAG270.24232633279483037No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC260.23335128343205885No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA260.23335128343205885No Hit
ACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTG260.23335128343205885No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT260.23335128343205885No Hit
GAGTTAGCACCCACCATTGGATGAAGCCCAGGATAAGTGACCCCCCCGGA260.23335128343205885No Hit
CATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCGGCAAT250.2243762340692874No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA250.2243762340692874No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG240.2154011847065159No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC240.2154011847065159No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG240.2154011847065159No Hit
GGATAAGTGACCCCCCCGGACCTTGGAGTTTCATGCTAATCAAAGAAGAG240.2154011847065159No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG230.20642613534374438No Hit
AGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCA230.20642613534374438No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG230.20642613534374438No Hit
GTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGTTAAATATCAA220.1974510859809729No Hit
TTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCC220.1974510859809729No Hit
CCATTGGATGAAGCCCAGGATAAGTGACCCCCCCGGACCTTGGAGTTTCA220.1974510859809729No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC220.1974510859809729No Hit
TGATTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCC220.1974510859809729No Hit
AGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTA220.1974510859809729No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA210.1884760366182014No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA210.1884760366182014No Hit
AAGGTGTACTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCA210.1884760366182014No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG210.1884760366182014No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG210.1884760366182014No Hit
GCGTTAGACCCCGTGACTCGTGGCTGAACGGCCGTACAACTCGACAGCCG210.1884760366182014No Hit
TAATATCCCTGCATCCATTATTTTCTAAAACTTTAACTGCTTCCCAAATT200.1795009872554299No Hit
CACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACT200.1795009872554299No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC200.1795009872554299No Hit
GGATCACATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTA200.1795009872554299No Hit
GCCTAAAGGTTCTTGCAGAGCAACATCATATACCCTGTAGAACCCGACTT190.1705259378926584No Hit
CCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTAT190.1705259378926584No Hit
GGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCCCTGCATCCA190.1705259378926584No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG190.1705259378926584No Hit
GTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGC190.1705259378926584No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC190.1705259378926584No Hit
GTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCT190.1705259378926584No Hit
GAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTT180.16155088852988692No Hit
GATGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCC180.16155088852988692No Hit
GAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATAT180.16155088852988692No Hit
GTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTT180.16155088852988692No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC180.16155088852988692No Hit
GAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATGGT170.15257583916711542No Hit
ATTAAAGAGTTAGCACCCACCATTGGATGAAGCCCAGGATAAGTGACCCC170.15257583916711542No Hit
GCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAAC170.15257583916711542No Hit
AGTTAGCACCCACCATTGGATGAAGCCCAGGATAAGTGACCCCCCCGGAC170.15257583916711542No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA170.15257583916711542No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC170.15257583916711542No Hit
CGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCC170.15257583916711542No Hit
GCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTTTGCTGGATA170.15257583916711542No Hit
CATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCA170.15257583916711542No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT160.14360078980434393No Hit
GCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGGCGCATAG160.14360078980434393No Hit
GCACCCACCATTGGATGAAGCCCAGGATAAGTGACCCCCCCGGACCTTGG160.14360078980434393No Hit
CCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTT160.14360078980434393No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA160.14360078980434393No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC160.14360078980434393No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA160.14360078980434393No Hit
GATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGAC160.14360078980434393No Hit
GTACTGGTAGGCGTTAGACCCCGTGACTCGTGGCTGAACGGCCGTACAAC160.14360078980434393No Hit
CTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAA150.1346257404415724No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG150.1346257404415724No Hit
GCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACATGAAAAAACAG150.1346257404415724No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA150.1346257404415724No Hit
CCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCC150.1346257404415724No Hit
GTCTCGACGGGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATT150.1346257404415724No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT150.1346257404415724No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG140.12565069107880095No Hit
ATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGA140.12565069107880095No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT140.12565069107880095No Hit
GCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAG140.12565069107880095No Hit
ATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGGATCACATTATG140.12565069107880095No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG140.12565069107880095No Hit
TGTATGGTCAGAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTT140.12565069107880095No Hit
CTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCT140.12565069107880095No Hit
GGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAA140.12565069107880095No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA140.12565069107880095No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA140.12565069107880095No Hit
TTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGGCGCATAGTCG130.11667564171602943No Hit
GCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGT130.11667564171602943No Hit
TGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTG130.11667564171602943No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC130.11667564171602943No Hit
TCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGAT130.11667564171602943No Hit
GTCCATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTT130.11667564171602943No Hit
ATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAA130.11667564171602943No Hit
GGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGG130.11667564171602943No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC130.11667564171602943No Hit
GATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACA130.11667564171602943No Hit
GTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATC130.11667564171602943No Hit
ATATCAGACATTCCTGTAGATAAAATCACCGGCTTGCCTGTTTTTGCCAC130.11667564171602943No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC130.11667564171602943No Hit
ACATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAA130.11667564171602943No Hit
GGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTG130.11667564171602943No Hit
CAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTT130.11667564171602943No Hit
GCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCT130.11667564171602943No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT130.11667564171602943No Hit
GCTTACAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGG120.10770059235325795No Hit
GGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATT120.10770059235325795No Hit
GCATCTCACAGTCACTGAAGGCTATTAAAGAGTTAGCACCCACCATTGGA120.10770059235325795No Hit
CCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATT120.10770059235325795No Hit
ACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGA120.10770059235325795No Hit
GCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCAC120.10770059235325795No Hit
CTCTTGCCGTGCGAGATGCTAGCAAAAAGGTGTACTTCTCAGCGGAGCAG120.10770059235325795No Hit
GCCCTAAATAGGATGCAGAGAATATCAAAGCGGAAAAAATCC120.10770059235325795No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC120.10770059235325795No Hit
CACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGA120.10770059235325795No Hit
GTACATGGGGGCTGGTGGCGTAGCTGTACGATACAGCC120.10770059235325795No Hit
CTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT120.10770059235325795No Hit
CTCGTAGACGGCATCTCACAGTCACTGAAGGCTATTAAAGAGTTAGCACC120.10770059235325795No Hit
AAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCCCGATG120.10770059235325795No Hit
GATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGT120.10770059235325795No Hit
GTATAATGTCTCGGACCCGTCGCAACGGTTGTTAATATGACAGGCCGCTA120.10770059235325795No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)