FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008170283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008170283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14913
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGGGGG1210.8113726279085363No Hit
TATCAACGCAGAGTACATGGGGGGG1210.8113726279085363No Hit
GTATCAACGCAGAGTACATGGGGGG1110.7443170388251861No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1110.7443170388251861No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1090.730905921008516No Hit
GGTATCAACGCAGAGTACATGGGGG1060.710789244283511No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA650.43586132904177566No Hit
GTATCAACGCAGAGTACATGGGGAG600.40233353450010056No Hit
GTATCAACGCAGAGTACATGGGGTG590.3956279755917656No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA500.3352779454167505No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT440.29504459196674043No Hit
GTATCAACGCAGAGTACATGGAGAG440.29504459196674043No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG440.29504459196674043No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC430.2883390330584054No Hit
GGTATCAACGCAGAGTACATGGGGGGG350.23469456179172535No Hit
TCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCCTGATTTT350.23469456179172535No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT340.22798900288339033No Hit
GTATCAACGCAGAGTACATGGGATG340.22798900288339033No Hit
GTATCAACGCAGAGTACATGGGAGG340.22798900288339033No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA340.22798900288339033No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT330.2212834439750553No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG330.2212834439750553No Hit
GGTATCAACGCAGAGTACATGGGGGG330.2212834439750553No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA320.2145778850667203No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA320.2145778850667203No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT310.2078723261583853No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC310.2078723261583853No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG300.20116676725005028No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG300.20116676725005028No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC300.20116676725005028No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG280.18775564943338027No Hit
GGTATCAACGCAGAGTACATGGGGTG240.16093341380004023No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT230.15422785489170523No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT230.15422785489170523No Hit
GGTATCAACGCAGAGTACATGGGAG220.14752229598337022No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT220.14752229598337022No Hit
TATCAACGCAGAGTACATGGGATTG210.1408167370750352No Hit
TATCAACGCAGAGTACATGGGGAAG200.1341111781667002No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA200.1341111781667002No Hit
GTATCAACGCAGAGTACATGGGGCG190.1274056192583652No Hit
GTATCAACGCAGAGTACATGGGATTG190.1274056192583652No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT190.1274056192583652No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA190.1274056192583652No Hit
TATCAACGCAGAGTACATGGGGAAAG190.1274056192583652No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT190.1274056192583652No Hit
GGTATCAACGCAGAGTACATGGAGAG190.1274056192583652No Hit
GGTATCAACGCAGAGTACATGGGGAG190.1274056192583652No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG190.1274056192583652No Hit
TATCAACGCAGAGTACATGGGAGTG180.12070006035003018No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA180.12070006035003018No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC180.12070006035003018No Hit
ACATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAA180.12070006035003018No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG180.12070006035003018No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC170.11399450144169516No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG170.11399450144169516No Hit
CAGTATATCCTCAGCAACTCTGCGTTGATACCACTGCTTCCGCGGACAGG170.11399450144169516Clontech Universal Primer Mix Long (96% over 25bp)
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA160.10728894253336015No Hit
GAGTATATAGAGAACAGCTAAAACTCAGCAGCAACAGCAACAACATTTCA160.10728894253336015No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT160.10728894253336015No Hit
GTACATGGGGGCTGGTGGCGTAGCTGTACGATACAGCC150.10058338362502514No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG150.10058338362502514No Hit
GCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAG150.10058338362502514No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC150.10058338362502514No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)