FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008170388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008170388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125669
Total Bases21.4 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTG4920.3915046670220977No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT4180.3326198187301562No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA4180.3326198187301562No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGA3390.2697562644725429No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC3380.2689605232794086No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG3330.2649818173137369No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2950.23474365197463176No Hit
GATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAA2590.20609696902179536No Hit
GCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCG2380.18938640396597411No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT2320.18461195680716805No Hit
AATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT2320.18461195680716805No Hit
AACCGAAGGTGATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGC2250.17904176845522762No Hit
AGATAGAAGTTTGAAGTGGAAAACTGGAAGACAGAAGTACGGGAAGGCGA2170.17267583891015287No Hit
GTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGATCTTTTAAAAA1790.14243767357104775No Hit
AGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAG1700.13527600283283864No Hit
CTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGATTAA1690.1344802616397043No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGA1620.12891007328776388No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1530.12174840254955477No Hit
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACAT1490.1185654377770174No Hit
AGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACGCAGGGAG1490.1185654377770174No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT1460.11617821419761436No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA1420.112995249425077No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1410.11219950823194265No Hit
GAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAA1340.10662931988000222No Hit
GGGAGAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGA1330.10583357868686788No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT1260.10026339033492747No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)