FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008170401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008170401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13710
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC3142.290299051787017No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC3132.2830051057622174No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1701.2399708242159009No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1220.8898614150255288No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT950.6929248723559446No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA950.6929248723559446No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT930.6783369803063457No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG920.6710430342815463No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA820.5981035740335521No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG800.5835156819839533No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA790.5762217359591539No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT740.5397520058351568No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT740.5397520058351568No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG730.5324580598103574No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA690.5032822757111598No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT670.48869438366156087No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC660.48140043763676155No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT630.4595185995623632No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG620.45222465353756386No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT600.437636761487965No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT600.437636761487965No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA590.43034281546316555No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT560.4084609773887673No Hit
CATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCGGCAAT550.401167031363968No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT550.401167031363968No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC550.401167031363968No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG520.37928519328956967No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG520.37928519328956967No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA490.3574033552151714No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG480.350109409190372No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG470.34281546316557254No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT460.33552151714077316No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG460.33552151714077316No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT450.3282275711159737No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG450.3282275711159737No Hit
GCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTT430.3136396790663749No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT430.3136396790663749No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA420.3063457330415755No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT420.3063457330415755No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG410.29905178701677604No Hit
ATATCAGACATTCCTGTAGATAAAATCACCGGCTTGCCTGTTTTTGCCAC400.29175784099197666No Hit
GCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCG390.2844638949671772No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG380.27716994894237784No Hit
GTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGC380.27716994894237784No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT370.2698760029175784No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA370.2698760029175784No Hit
CTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAATT370.2698760029175784No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC370.2698760029175784No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC360.26258205689277897No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC360.26258205689277897No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT360.26258205689277897No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC360.26258205689277897No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG360.26258205689277897No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC360.26258205689277897No Hit
CATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAGCT350.2552881108679796No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA340.24799416484318015No Hit
GTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGTTAAATATCAA330.24070021881838077No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC330.24070021881838077No Hit
GAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTT310.22611232676878193No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT310.22611232676878193No Hit
GGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGCTTATGA300.2188183807439825No Hit
TCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACA300.2188183807439825No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA300.2188183807439825No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC290.21152443471918309No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG290.21152443471918309No Hit
GCGTTAGACCCCGTGACTCGTGGCTGAACGGCCGTACAACTCGACAGCCG280.20423048869438365No Hit
GTACTGGTAGGCGTTAGACCCCGTGACTCGTGGCTGAACGGCCGTACAAC280.20423048869438365No Hit
TTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCC270.19693654266958427No Hit
GATGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCC270.19693654266958427No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG270.19693654266958427No Hit
ATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAAA260.18964259664478483No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC260.18964259664478483No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC260.18964259664478483No Hit
CTACTGGGGCGAGTATTCCCAATGTATGGTCAGAATATCCCACAGGGATA260.18964259664478483No Hit
CTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTC250.18234865061998543No Hit
GATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGAC250.18234865061998543No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA240.175054704595186No Hit
AATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGT240.175054704595186No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC240.175054704595186No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA240.175054704595186No Hit
ACATTGATTGCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGA230.16776075857038658No Hit
GCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAG230.16776075857038658No Hit
CTTCAAATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTT230.16776075857038658No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG230.16776075857038658No Hit
GGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAAT220.16046681254558717No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG220.16046681254558717No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC220.16046681254558717No Hit
CTTTAACTGCTTCCCAAATTTCCCCAATATCAGACATTCCTGTAGATAAA220.16046681254558717No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA220.16046681254558717No Hit
TCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACT220.16046681254558717No Hit
CTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCT220.16046681254558717No Hit
GCTTGAGGAGCTTGAAGCAGATGATATCCCGATGCTGACGGCTTACAATA210.15317286652078774No Hit
ATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAA210.15317286652078774No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC210.15317286652078774No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG210.15317286652078774No Hit
GTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCT210.15317286652078774No Hit
GCTCCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAG210.15317286652078774No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC210.15317286652078774No Hit
CTATTAAAGAGTTAGCACCCACCATTGGATGAAGCCCAGGATAAGTGACC200.14587892049598833No Hit
GTCCATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTT200.14587892049598833No Hit
ATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAAAACTTT200.14587892049598833No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG200.14587892049598833No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA200.14587892049598833No Hit
CTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCG200.14587892049598833No Hit
GGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCTGAGGGACTCA200.14587892049598833No Hit
GTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTT200.14587892049598833No Hit
ATGTAGGAGAGAGAGGTTAATAAGGCCTAGCCTAAAGGTTCTTGCAGAGC200.14587892049598833No Hit
AGTTTACTGCGTCACGCTGAGGGACTCATCCTGGGCTACAATCCTATTGC200.14587892049598833No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC200.14587892049598833No Hit
AACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGA190.13858497447118892No Hit
GCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTTTGCTGGATA190.13858497447118892No Hit
CACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGA190.13858497447118892No Hit
ACATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAA190.13858497447118892No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC190.13858497447118892No Hit
GATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACA180.13129102844638948No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC180.13129102844638948No Hit
TGATTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCC180.13129102844638948No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC180.13129102844638948No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT180.13129102844638948No Hit
GCTTGAAGCAGATGATATCCCGATGCTGACGGCTTACAATAAACGTGATC170.12399708242159008No Hit
TTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGGCGCATAGTCG170.12399708242159008No Hit
GCTACTAGTTTACTGCGTCACGCTGAGGGACTCATCCTGGGCTACAATCC170.12399708242159008No Hit
TCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGG170.12399708242159008No Hit
CGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCTGTTTGCCACGCT170.12399708242159008No Hit
CGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCC170.12399708242159008No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC160.11670313639679065No Hit
GGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCCCTGCATCCA160.11670313639679065No Hit
ATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCA160.11670313639679065No Hit
GTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACA160.11670313639679065No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG160.11670313639679065No Hit
ATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGG160.11670313639679065No Hit
CCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCC160.11670313639679065No Hit
CATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCA160.11670313639679065No Hit
TATCAACGCAGAGTACATGGGGGCTGGTGGCGTAGCTGTACGATACAGCC160.11670313639679065No Hit
CATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCA160.11670313639679065No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG160.11670313639679065No Hit
GATCATGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCAT150.10940919037199125No Hit
TCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCCTGATTTT150.10940919037199125No Hit
GTACATGGGCGTCAATCGAAAGATATGACG150.10940919037199125No Hit
GTAGAGTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGTTAAAT150.10940919037199125No Hit
GTGTATAATGTCTCGGACCCGTCGCAACGGTTGTTAATATGACAGGCCGC150.10940919037199125No Hit
AAGCAGATGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAA140.10211524434719182No Hit
TCCTTATCTTGAGACGCATAGCAAATGTAGGAGAGAGAGGTTAATAAGGC140.10211524434719182No Hit
CTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAA140.10211524434719182No Hit
GTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCTGTTTG140.10211524434719182No Hit
TGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAAC140.10211524434719182No Hit
GTAAAATGGTGAAGCTTATGAATTTGTGGAGTGAGAGGATTAAAGATAGG140.10211524434719182No Hit
TCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCCCT140.10211524434719182No Hit
CTTTAAAGAAGCGATTCAGCGCTATTTGCGCCAAGAACTGTTAACGTCTT140.10211524434719182No Hit
CTCTTGCCGTGCGAGATGCTAGCAAAAAGGTGTACTTCTCAGCGGAGCAG140.10211524434719182No Hit
GCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTT140.10211524434719182No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA140.10211524434719182No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)