FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008170658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008170658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17109
Total Bases2.6 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1751.0228534689344788No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1300.7598340054941843No Hit
GTACATGGGCGTCATATCGAAAGATATGACG1190.6955403588754456No Hit
GTACATGGGCGTCATATCGAAAGATATG990.578642819568648No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC870.5085042959845696No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG820.47927991115787016No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA570.33315798702437316No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT540.31562335612835346No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT510.2980887252323339No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG500.292243848266994No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG490.2863989713016541No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA460.26886434040563445No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC390.22795020164825527No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA390.22795020164825527No Hit
GAGTACATGGGCGTCATATCGAAAGATATGACG380.2221053246829154No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC380.2221053246829154No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT370.21626044771757552No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC360.21041557075223566No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC350.2045706937868958No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG320.18703606289087613No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC310.18119118592553626No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT310.18119118592553626No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG310.18119118592553626No Hit
GGTATCAACGCAGAGTACATGGGGG300.1753463089601964No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG300.1753463089601964No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA300.1753463089601964No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT290.1695014319948565No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA280.16365655502951662No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT270.15781167806417673No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC260.15196680109883687No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG260.15196680109883687No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG250.146121924133497No Hit
GAGTACATGGGCGTCATATCGAAAGATATG250.146121924133497No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC230.13443217020281722No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT230.13443217020281722No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGTGCA230.13443217020281722No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA220.12858729323747736No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT220.12858729323747736No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT220.12858729323747736No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA220.12858729323747736No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA220.12858729323747736No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATG220.12858729323747736No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTG220.12858729323747736No Hit
GACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGC210.12274241627213747No Hit
GCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAA210.12274241627213747No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG210.12274241627213747No Hit
CCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA210.12274241627213747No Hit
CTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGCATTACT200.1168975393067976No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG200.1168975393067976No Hit
ATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAA200.1168975393067976No Hit
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG200.1168975393067976No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA200.1168975393067976No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG200.1168975393067976No Hit
GGGTAAGATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATT200.1168975393067976No Hit
TGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGC200.1168975393067976No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT200.1168975393067976No Hit
GTATCAACGCAGAGTACATGGGGGG190.1110526623414577No Hit
TCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGT190.1110526623414577No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG190.1110526623414577No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG190.1110526623414577No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG190.1110526623414577No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG190.1110526623414577No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT190.1110526623414577No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG190.1110526623414577No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC190.1110526623414577No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG180.10520778537611783No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA180.10520778537611783No Hit
CACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGA180.10520778537611783No Hit
GCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAG180.10520778537611783No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA180.10520778537611783No Hit

[OK]Adapter Content

Adapter graph