FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008170763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008170763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20111
Total Bases3 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC3051.5165829645467654No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2611.2977972253990353No Hit
GTACATGGGCGTCATATCGAAAGATATGACG1340.6663020237680871No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1220.6066331858187062No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1180.5867435731689125No Hit
GTACATGGGCGTCATATCGAAAGATATG940.4674058972701507No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC890.44254388145790857No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG870.43259907513301177No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA870.43259907513301177No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA820.40773705932076976No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA760.37790264034607923No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT560.2784545770971111No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA510.25359256128486896No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT510.25359256128486896No Hit
GGTATCAACGCAGAGTACATGGGGG500.24862015812242058No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT480.23867535179752375No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG470.23370294863507535No Hit
GAGTACATGGGCGTCATATCGAAAGATATGACG460.22873054547262692No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC460.22873054547262692No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG460.22873054547262692No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG420.20884093282283328No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC400.19889612649793645No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG400.19889612649793645No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT390.19392372333548805No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG390.19392372333548805No Hit
GTATCAACGCAGAGTACATGGGGGG360.1790065138481428No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG360.1790065138481428No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC360.1790065138481428No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA360.1790065138481428No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG350.17403411068569438No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT330.16408930436079758No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC330.16408930436079758No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC330.16408930436079758No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC320.15911690119834915No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG320.15911690119834915No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT310.15414449803590075No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT300.14917209487345234No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA300.14917209487345234No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT300.14917209487345234No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC300.14917209487345234No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA290.1441996917110039No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG290.1441996917110039No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC290.1441996917110039No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT280.13922728854855554No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC280.13922728854855554No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA280.13922728854855554No Hit
GAGTACATGGGCGTCATATCGAAAGATATG280.13922728854855554No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG280.13922728854855554No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC260.12928248222365868No Hit
CGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTC250.12431007906121029No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT240.11933767589876187No Hit
GTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTT240.11933767589876187No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATG240.11933767589876187No Hit
GCTTGAAGCAGATGATATCCCGATGCTGACGGCTTACAATAAACGTGATC230.11436527273631346No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA230.11436527273631346No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT230.11436527273631346No Hit
GTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCT230.11436527273631346No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC220.10939286957386506No Hit
GTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCCCGATGCTGAC220.10939286957386506No Hit
CTTCAAATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTT220.10939286957386506No Hit
CATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCA220.10939286957386506No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC220.10939286957386506No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT220.10939286957386506No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT220.10939286957386506No Hit
GCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCT210.10442046641141664No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG210.10442046641141664No Hit
CACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACT210.10442046641141664No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT210.10442046641141664No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC210.10442046641141664No Hit
GCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAG210.10442046641141664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)