FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008170772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008170772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18512
Total Bases2.7 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2031.0965859982713915No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1931.0425669835782196No Hit
GTACATGGGCGTCATATCGAAAGATATGACG1730.9345289541918756No Hit
GTACATGGGCGTCATATCGAAAGATATG910.49157303370786515No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC860.4645635263612792No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA760.4105445116681072No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA750.40514261019878994No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT660.3565254969749352No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA650.35112359550561795No Hit
GGTATCAACGCAGAGTACATGGGGG590.31871218668971474No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA540.29170267934312877No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC520.2808988764044944No Hit
GTATCAACGCAGAGTACATGGGGGG510.2754969749351772No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG500.27009507346586No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG490.2646931719965428No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG470.2538893690579084No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG470.2538893690579084No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT430.2322817631806396No Hit
GAGTACATGGGCGTCATATCGAAAGATATGACG430.2322817631806396No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG410.2214779602420052No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT410.2214779602420052No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT400.216076058772688No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG360.19446845289541917No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG340.1836646499567848No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT330.1782627484874676No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG320.17286084701815038No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC310.1674589455488332No Hit
GAGTACATGGGCGTCATATCGAAAGATATG310.1674589455488332No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC300.162057044079516No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC290.15665514261019878No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA290.15665514261019878No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG290.15665514261019878No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA290.15665514261019878No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA280.1512532411408816No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA280.1512532411408816No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT280.1512532411408816No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA280.1512532411408816No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC270.14585133967156438No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG260.1404494382022472No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC260.1404494382022472No Hit
AGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCA260.1404494382022472No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT250.13504753673293No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT250.13504753673293No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG250.13504753673293No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT240.12964563526361278No Hit
CTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCG240.12964563526361278No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG240.12964563526361278No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA240.12964563526361278No Hit
GCTCCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAG240.12964563526361278No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC240.12964563526361278No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC230.12424373379429558No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT230.12424373379429558No Hit
GATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACA220.11884183232497839No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATG220.11884183232497839No Hit
CGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCC220.11884183232497839No Hit
TGATTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCC210.1134399308556612No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC210.1134399308556612No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT210.1134399308556612No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG200.108038029386344No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT200.108038029386344No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATG200.108038029386344No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG200.108038029386344No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG200.108038029386344No Hit
GTATCAACGCAGAGTACATGGGGAG190.1026361279170268No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT190.1026361279170268No Hit
GGTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG190.1026361279170268No Hit
TCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGCCG190.1026361279170268No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG190.1026361279170268No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT190.1026361279170268No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA190.1026361279170268No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC190.1026361279170268No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATG190.1026361279170268No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)