FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008170816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008170816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93919
Total Bases15.8 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG5810.6186181709771186No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC5530.5888052470746068No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC4830.5142729373183275No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG4290.4567765840777691No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC3460.3684025596524665No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA3160.3364601411854896No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC3100.3300716574920942No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA3070.3268774156453966No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA2990.3183594373875361No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC2860.30451772271851274No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC2550.2715105569693033No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA2500.2661868205581405No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG2480.26405732599367543No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC2280.24276238034902417No Hit
GCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAA2280.24276238034902417No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC2250.23956813850232647No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA2220.2363738966556288No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA2180.23211490752669856No Hit
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG2120.22572642383330319No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA2050.2182731928576752No Hit
TCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGT1850.196978247213024No Hit
GTACATGGGCGTCATATCGAAAGATATGACG1840.1959134999307914No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA1830.19484875264855886No Hit
CGTAAAGACGTTAGGTCAAGGTGTAGCCCATGAGGTGGCAAGAAATGGGC1820.1937840053663263No Hit
ATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCA1810.19271925808409376No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA1810.19271925808409376No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT1780.18952501623739607No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA1670.17781279613283787No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA1660.17674804885060533No Hit
GGTGTAGCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAA1640.17461855428614018No Hit
GTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAG1640.17461855428614018No Hit
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC1610.1714243124394425No Hit
CCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCG1600.17035956515720996No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1580.1682300705927448No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG1570.16716532331051226No Hit
GTGTAGCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAA1570.16716532331051226No Hit
ACTGAGAGTAGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACC1560.16610057602827968No Hit
CCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCTACTTCTAACC1560.16610057602827968No Hit
GTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCT1540.16397108146381456No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA1530.162906334181582No Hit
GCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAA1530.162906334181582No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT1520.16184158689934944No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC1520.16184158689934944No Hit
GCATAATATTTCGCCCACTAAGCCAATCACTTTATTGACTCCTAGCCGCA1500.15971209233488431No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT1500.15971209233488431No Hit
CTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGCATTACT1450.15438835592372152No Hit
CCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGA1450.15438835592372152No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG1400.1490646195125587No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC1400.1490646195125587No Hit
GTACATGGGCGTCATATCGAAAGATATG1370.145870377665861No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1360.14480563038362845No Hit
CCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACT1360.14480563038362845No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAG1320.1405466412546982No Hit
TAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCA1320.1405466412546982No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT1300.13841714669023308No Hit
CGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAA1300.13841714669023308No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT1280.13628765212576793No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT1270.13522290484353539No Hit
CCCTATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATA1260.13415815756130284No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCA1250.13309341027907026No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA1240.13202866299683771No Hit
CTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCT1230.13096391571460514No Hit
ATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCA1230.13096391571460514No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT1230.13096391571460514No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA1180.12564017930344235No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT1160.1235106847389772No Hit
CCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACG1140.12138119017451209No Hit
CCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTA1130.12031644289227951No Hit
GAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAAC1120.11925169561004696No Hit
GGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG1120.11925169561004696No Hit
GCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC1110.1181869483278144No Hit
CCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAA1110.1181869483278144No Hit
GCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATC1090.11605745376334928No Hit
GGTCAAGGTGTAGCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACC1090.11605745376334928No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG1090.11605745376334928No Hit
CCTTAACCTCTACTTCTACCTACGCCTAATCTACTCCACCTCAATCACAC1080.1149927064811167No Hit
GTCAAGGTGTAGCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCC1070.11392795919888414No Hit
GTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAG1070.11392795919888414No Hit
CCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAA1060.11286321191665159No Hit
CCATATATCCAAACAACAAAGCATAATATTTCGCCCACTAAGCCAATCAC1050.11179846463441902No Hit
GTACTATACTTCACAACAATCCTAATCCTAATACCAACTATCTCCCTAAT1050.11179846463441902No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC1040.11073371735218646No Hit
ACACAATGGGGCTCACTCACCCACCACATTAACAACATAAAACCCTCATT1030.10966897006995391No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA1030.10966897006995391No Hit
CTTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATACCCCCGATTCCG1030.10966897006995391No Hit
CTATATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATACAT1020.10860422278772133No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG1000.10647472822325622No Hit
GCAGTAAACTGAGAGTAGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCGT1000.10647472822325622No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT1000.10647472822325622No Hit
CTACACAACATATTTTGTCACCAAGACCCTACTTCTAACCTCCCTGTTCT990.10540998094102365No Hit
ACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA980.10434523365879109No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA980.10434523365879109No Hit
CCTCACCACCTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCTG970.10328048637655854No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCC960.10221573909432596No Hit
CCCTGATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAG960.10221573909432596No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTG960.10221573909432596No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT960.10221573909432596No Hit
TAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACAC940.10008624452986084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)