FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008170861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008170861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40173
Total Bases6 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC3830.9533766460060239No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC3720.9259950713165559No Hit
GTACATGGGCGTCATATCGAAAGATATGACG3190.7940656659945734No Hit
GTACATGGGCGTCATATCGAAAGATATG2660.662136260672591No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1560.38832051377791055No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1330.3310681303362955No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1240.3086650237721853No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG1100.2738157468946805No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG1100.2738157468946805No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG1060.2638588106439649No Hit
GAGTACATGGGCGTCATATCGAAAGATATGACG1000.24892340626789136No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG970.24145570407985462No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG950.23647723595449677No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC920.22900953376646005No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC900.22403106564110226No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA890.22154183157842333No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT880.2190525975157444No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG860.21407412939038659No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA820.20411719313967094No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA800.1991387250143131No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT790.19664949095163417No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA780.19416025688895527No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA770.19167102282627635No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTG700.17424638438752396No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA680.1692679162621661No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT680.1692679162621661No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG680.1692679162621661No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT660.1642894481368083No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC650.1618002140741294No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG640.15931098001145047No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC640.15931098001145047No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT620.15433251188609265No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA610.15184327782341375No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT610.15184327782341375No Hit
CCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT600.14935404376073483No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC580.14437557563537698No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA580.14437557563537698No Hit
GGTATCAACGCAGAGTACATGGGGG580.14437557563537698No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG570.14188634157269808No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA570.14188634157269808No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT560.13939710751001916No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT550.13690787344734026No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA540.13441863938466134No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG540.13441863938466134No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA540.13441863938466134No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT510.1269509371966246No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA510.1269509371966246No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATG500.12446170313394568No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC490.12197246907126677No Hit
CCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGG480.11948323500858785No Hit
GTATCAACGCAGAGTACATGGGGGG470.11699400094590895No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATG470.11699400094590895No Hit
ATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCC460.11450476688323002No Hit
GAGTACATGGGCGTCATATCGAAAGATATG460.11450476688323002No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC460.11450476688323002No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA460.11450476688323002No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG450.11201553282055113No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG440.1095262987578722No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT440.1095262987578722No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG430.10703706469519329No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC430.10703706469519329No Hit
CCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATC430.10703706469519329No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATG430.10703706469519329No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT420.10454783063251437No Hit
CTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCG410.10205859656983547No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG410.10205859656983547No Hit
CACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACT410.10205859656983547No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)