FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008170877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008170877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18094
Total Bases2.4 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGCGTCATATCGAAAGATATGACG2241.2379794406985742No Hit
GTACATGGGCGTCATATCGAAAGATATG1931.0666519288161822No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC990.5471426992373163No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC960.5305626174422461No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG830.45871559633027525No Hit
TATCAACGCAGAGTACATGGGGGGG810.4476622084668951No Hit
GTATCAACGCAGAGTACATGGGGGGG750.41450204487675474No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG710.39239526914999445No Hit
GAGTACATGGGCGTCATATCGAAAGATATG670.3702884934232342No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATG610.3371283298330939No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG590.3260749419697137No Hit
GAGTACATGGGCGTCATATCGAAAGATATGACG570.3150215541063336No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATG540.2984414723112634No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATG530.2929147783795733No Hit
GGTATCAACGCAGAGTACATGGGGG500.27633469658450316No Hit
GTATCAACGCAGAGTACATGGGGGG490.2708080026528131No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA480.26528130872112304No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA460.2542279208577429No Hit
GTATCAACGCAGAGTACATGGGGAG430.23764783906267273No Hit
GTATCAACGCAGAGTACATGGGGTG420.23212114513098264No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC340.18790759367746215No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG320.176854205814082No Hit
GTATCAACGCAGAGTACATGGGAGG320.176854205814082No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG290.1602741240190118No Hit
GTATCAACGCAGAGTACATGGAGAG280.15474743008732178No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG280.15474743008732178No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT280.15474743008732178No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC280.15474743008732178No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT250.13816734829225158No Hit
TATCAACGCAGAGTACATGGGAGAG250.13816734829225158No Hit
GGTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG250.13816734829225158No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT240.13264065436056152No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC240.13264065436056152No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC240.13264065436056152No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG240.13264065436056152No Hit
GGTATCAACGCAGAGTACATGGGGGGG230.12711396042887144No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT230.12711396042887144No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA220.12158726649718139No Hit
GCTTGAAGCAGATGATATCCCGATGCTGACGGCTTACAATAAACGTGATC210.11606057256549132No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA210.11606057256549132No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA210.11606057256549132No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT200.11053387863380126No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG190.10500718470211119No Hit
TATCAACGCAGAGTACATGGGACTG190.10500718470211119No Hit
ATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG190.10500718470211119No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA190.10500718470211119No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC190.10500718470211119No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)