FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008170883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008170883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30817
Total Bases4 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGCGTCATATCGAAAGATATGACG1850.6003180062952267No Hit
GTACATGGGCGTCATATCGAAAGATATG1640.532173800175228No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC960.3115163708342798No Hit
GGTATCAACGCAGAGTACATGGGGG770.24986208910666188No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG770.24986208910666188No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC770.24986208910666188No Hit
GTATCAACGCAGAGTACATGGGGGG740.24012720251809067No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG740.24012720251809067No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA720.23363727812570984No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA680.22065742934094817No Hit
GAGTACATGGGCGTCATATCGAAAGATATGACG670.21741246714475776No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG650.21092254275237693No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG620.20118765616380568No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC530.17198299639809195No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG510.16549307200571114No Hit
TATCAACGCAGAGTACATGGGGGGG500.1622481098095207No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC500.1622481098095207No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT480.1557581854171399No Hit
GAGTACATGGGCGTCATATCGAAAGATATG470.15251322322094946No Hit
GTATCAACGCAGAGTACATGGGGTG470.15251322322094946No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG460.14926826102475907No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA450.14602329882856865No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC450.14602329882856865No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATG450.14602329882856865No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTG420.1362884122399974No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA410.133043450043807No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATG410.133043450043807No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG410.133043450043807No Hit
GTATCAACGCAGAGTACATGGGGGGG390.12655352565142616No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT390.12655352565142616No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA390.12655352565142616No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC380.12330856345523573No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC370.12006360125904533No Hit
GTATCAACGCAGAGTACATGGGGAG360.11681863906285492No Hit
CCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGG360.11681863906285492No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA360.11681863906285492No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA350.11357367686666452No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA330.10708375247428367No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA310.10059382808190284No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATG310.10059382808190284No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)