FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008170953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008170953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92824
Total Bases15.1 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC7840.844609152805309No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG5460.5882099457036973No Hit
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG4610.49663880031026464No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC4050.43630957510988544No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC3800.4093768852882875No Hit
GTACATGGGCGTCATATCGAAAGATATGACG2850.30703266396621565No Hit
GCATAATATTTCGCCCACTAAGCCAATCACTTTATTGACTCCTAGCCGCA2840.30595535637335175No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG2770.2984142032233043No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA2740.29518228044471256No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC2730.29410497285184867No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA2640.28440920451607343No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC2370.2553218995087478No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA2260.2434715159872447No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG2220.239162285615789No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAG2180.23485305524433334No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA2080.22407997931569423No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA2050.2208480565371025No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA2040.21977074894423856No Hit
CCCTATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATA1960.21115228820132725No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA1950.21007498060846333No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC1920.20684305782987158No Hit
GTACATGGGCGTCATATCGAAAGATATG1920.20684305782987158No Hit
GCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAA1900.20468844264414374No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1880.20253382745841594No Hit
CCATATATCCAAACAACAAAGCATAATATTTCGCCCACTAAGCCAATCAC1870.20145651986555202No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA1850.1993019046798242No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1830.19714728949409635No Hit
CCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGA1830.19714728949409635No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA1750.18852882875118504No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA1710.1842195983797294No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT1610.17344652245109024No Hit
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC1600.17236921485822632No Hit
TCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGT1570.1691372920796346No Hit
CCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCTACTTCTAACC1560.16805998448677067No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1550.16698267689390675No Hit
CCTTAACCTCTACTTCTACCTACGCCTAATCTACTCCACCTCAATCACAC1520.163750754115315No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA1500.16159613892958719No Hit
CCTTACGAGTGCGGCTTCGACCCTATATCCCCCGCCCGCGTCCCTTTCTC1440.1551322933724037No Hit
GTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT1400.15082306300094803No Hit
CCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCACCC1300.1400499870723089No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC1290.13897267947944497No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT1280.13789537188658108No Hit
GGTATCAACGCAGAGTACATGGGGG1280.13789537188658108No Hit
GCTATTACCTTCTTATTATTTGATCTAGAAATTGCCCTCCTTTTACCCCT1270.13681806429371715No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA1240.13358614151512538No Hit
GTATCAACGCAGAGTACATGGGGGG1240.13358614151512538No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA1240.13358614151512538No Hit
CTATATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATACAT1230.13250883392226148No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA1230.13250883392226148No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA1220.13143152632939759No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT1220.13143152632939759No Hit
CGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACC1220.13143152632939759No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT1210.13035421873653366No Hit
ACACAATGGGGCTCACTCACCCACCACATTAACAACATAAAACCCTCATT1180.12712229595794192No Hit
CCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAA1180.12712229595794192No Hit
CCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACT1180.12712229595794192No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG1170.126044988365078No Hit
GTACTATACTTCACAACAATCCTAATCCTAATACCAACTATCTCCCTAAT1140.12281306558648625No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG1130.12173575799362235No Hit
CCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAAC1130.12173575799362235No Hit
CTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGCATTACT1130.12173575799362235No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC1110.1195811428078945No Hit
TAGTTATGTCATCCCTCTTATTAATCATCATCCTAGCCCTAAGTCTGGCC1100.11850383521503059No Hit
CCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA1100.11850383521503059No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT1090.11742652762216667No Hit
CACTAAGCCAATCACTTTATTGACTCCTAGCCGCAGACCTCCTCATTCTA1080.11634922002930277No Hit
CTATATCCCCCGCCCGCGTCCCTTTCTCCATAAAATTCTTCTTAGTAGCT1070.11527191243643886No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA1060.11419460484357494No Hit
CCATAAACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTC1050.11311729725071101No Hit
ATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCA1040.11203998965784712No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA1040.11203998965784712No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT1040.11203998965784712No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1020.10988537447211928No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC1020.10988537447211928No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC1000.10773075928639145No Hit
AACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTCGAAGA1000.10773075928639145No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG990.10665345169352755No Hit
ACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGG990.10665345169352755No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA980.10557614410066363No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG980.10557614410066363No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG980.10557614410066363No Hit
CTTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATACCCCCGATTCCG970.1044988365077997No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT950.10234422132207187No Hit
CAACTAACCTGCCACTAATAGTTATGTCATCCCTCTTATTAATCATCATC950.10234422132207187No Hit
GTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAAT940.10126691372920797No Hit
GAGTACATGGGCGTCATATCGAAAGATATGACG930.10018960613634405No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)