FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008170963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008170963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61636
Total Bases9.4 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC3880.6295022389512622No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC3500.5678499578168602No Hit
GTACATGGGCGTCATATCGAAAGATATGACG2650.4299435394899085No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1780.28879226426114607No Hit
GTACATGGGCGTCATATCGAAAGATATG1480.24011941073398663No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1420.23038484002855475No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA1320.21416055551950158No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT1240.20118112791225906No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC1190.19306898565773248No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1180.19144655720682718No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA1090.17684470114867934No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA1070.17359984424686872No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1060.1719774157959634No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT1050.17035498734505808No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG1040.16873255889415276No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC1030.16711013044324746No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG880.14277370367966774No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG860.1395288467778571No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA850.13790641832695177No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA810.1314167045233305No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG800.12979427607242522No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA790.1281718476215199No Hit
GAGTACATGGGCGTCATATCGAAAGATATGACG760.12330456226880394No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG750.12168213381789862No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC700.11356999156337205No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA700.11356999156337205No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA700.11356999156337205No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA690.11194756311246674No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG690.11194756311246674No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT670.10870270621065611No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT670.10870270621065611No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT660.10708027775975079No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG640.10383542085794016No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC640.10383542085794016No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG620.10059056395612953No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC620.10059056395612953No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)