FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008170964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008170964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38630
Total Bases5.6 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4511.167486409526275No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC4081.056173958063681No Hit
GTACATGGGCGTCATATCGAAAGATATGACG2740.7092932953662956No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1560.4038312192596428No Hit
GTACATGGGCGTCATATCGAAAGATATG1460.3779446026404349No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1440.3727672793165933No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1430.3701786176546725No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT1190.30805073776857367No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA1180.3054620761066528No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG1020.26404348951592027No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1010.2614548278539995No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG920.2381568728967124No Hit
GAGTACATGGGCGTCATATCGAAAGATATGACG910.2355682112347916No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC870.22521356458710845No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG840.2174475796013461No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT780.2019156096298214No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT780.2019156096298214No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA750.19414962464405902No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT710.18379497799637587No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG660.17085166968677193No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC660.17085166968677193No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG640.16567434636293035No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC630.16308568470100956No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT630.16308568470100956No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA620.16049702303908878No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT580.15014237639140562No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG580.15014237639140562No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT570.14755371472948484No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG570.14755371472948484No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG570.14755371472948484No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG540.1397877297437225No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA530.1371990680818017No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG530.1371990680818017No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG520.13461040641988092No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG510.13202174475796014No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA500.12943308309603935No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG490.12684442143411856No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT490.12684442143411856No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT470.12166709811027698No Hit
GGTATCAACGCAGAGTACATGGGGG470.12166709811027698No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA460.1190784364483562No Hit
GCCTAAAGGTTCTTGCAGAGCAACATCATATACCCTGTAGAACCCGACTT460.1190784364483562No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG460.1190784364483562No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG460.1190784364483562No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT450.11648977478643542No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT450.11648977478643542No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATG430.11131245146259384No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG430.11131245146259384No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC430.11131245146259384No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG420.10872378980067306No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC420.10872378980067306No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC420.10872378980067306No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA420.10872378980067306No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC400.1035464664768315No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATG400.1035464664768315No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC390.1009578048149107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)