FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008170997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008170997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27592
Total Bases4 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2280.8263264714409974No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2110.7647144099739055No Hit
GTACATGGGCGTCATATCGAAAGATATGACG1760.6378660481298928No Hit
GTACATGGGCGTCATATCGAAAGATATG1540.5581327921136562No Hit
GGTATCAACGCAGAGTACATGGGGG1200.43490866917947235No Hit
GTATCAACGCAGAGTACATGGGGGG1120.4059147579008408No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1120.4059147579008408No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA900.3261815018846042No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC860.3116845462452885No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA850.3080603073354596No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC780.28269063496665703No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA680.24644824586836767No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA670.24282400695853873No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT650.23557552913888083No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA600.21745433458973618No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT590.21383009567990724No Hit
GTCTTGGAGAGAGCAACTCTGCGTTGATACCACTGCTTCCGCGGACAGGC580.2102058567700783Clontech Universal Primer Mix Long (96% over 25bp)
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA560.2029573789504204No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG550.19933314004059147No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG540.19570890113076253No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG530.1920846622209336No Hit
GGTATCAACGCAGAGTACATGGGGGG520.18846042331110469No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA510.18483618440127572No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC500.18121194549144679No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG500.18121194549144679No Hit
GAGTACATGGGCGTCATATCGAAAGATATGACG490.17758770658161785No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG490.17758770658161785No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA480.17396346767178894No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA470.17033922876196No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG450.1630907509423021No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA450.1630907509423021No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT440.1594665120324732No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA430.15584227312264426No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG430.15584227312264426No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT420.1522180342128153No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT420.1522180342128153No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATG420.1522180342128153No Hit
CGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACC410.14859379530298636No Hit
CCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGA400.14496955639315745No Hit
CCCTAGCATTACTTATATGATATGTCTCCATACCCATTACAATCTCCAGC400.14496955639315745No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG400.14496955639315745No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG390.14134531748332851No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT390.14134531748332851No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT380.13772107857349958No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG370.13409683966367061No Hit
GCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAA370.13409683966367061No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG370.13409683966367061No Hit
GAGTACATGGGCGTCATATCGAAAGATATG360.1304726007538417No Hit
CTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGCATTACT360.1304726007538417No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA350.12684836184401277No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC350.12684836184401277No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC340.12322412293418383No Hit
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG330.11959988402435488No Hit
GCTCCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAG330.11959988402435488No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT310.11235140620469701No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG310.11235140620469701No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC300.10872716729486809No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATG300.10872716729486809No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG300.10872716729486809No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC290.10510292838503915No Hit
GCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAAC290.10510292838503915No Hit
GCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGT280.1014786894752102No Hit
ACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGG280.1014786894752102No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC280.1014786894752102No Hit
GTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT280.1014786894752102No Hit
CGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCC280.1014786894752102No Hit
CCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA280.1014786894752102No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA280.1014786894752102No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)