FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008171000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008171000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72175
Total Bases12.3 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG8221.1388985105646No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4530.6276411499826811No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC4420.6124004156563907No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC3920.5431243505368895No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC3620.5015587114651888No Hit
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG2530.35053688950467615No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA2190.3034291652234153No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG2080.28818843089712504No Hit
CCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGA1990.27571873917561485No Hit
GCATAATATTTCGCCCACTAAGCCAATCACTTTATTGACTCCTAGCCGCA1900.2632490474541046No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG1890.2618635261517146No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC1810.2507793557325944No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG1790.24800831312781435No Hit
CGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACC1750.24246622791825423No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1730.23969518531347422No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA1650.22861101489435398No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA1620.22445445098718395No Hit
GCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAG1610.22306892968479392No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA1570.2175268444752338No Hit
GACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGC1570.2175268444752338No Hit
CCCTATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATA1550.21475580187045373No Hit
CCATATATCCAAACAACAAAGCATAATATTTCGCCCACTAAGCCAATCAC1450.20090058884655349No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1440.19951506754416348No Hit
CCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAA1430.19812954624177348No Hit
GCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAA1420.19674402493938345No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA1410.19535850363699342No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT1390.19258746103221336No Hit
CCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAG1380.19120193972982336No Hit
ACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGG1320.1828888119154832No Hit
GTACATGGGCGTCATATCGAAAGATATGACG1290.17873224800831314No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA1290.17873224800831314No Hit
CCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA1270.17596120540353308No Hit
ATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAA1240.17180464149636301No Hit
CTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGCATTACT1180.16349151368202286No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA1170.16210599237963283No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA1150.1593349497748528No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT1140.15794942847246277No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT1090.15102182196051264No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1090.15102182196051264No Hit
CCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCTACTTCTAACC1080.1496363006581226No Hit
AGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAA1070.14825077935573258No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT1070.14825077935573258No Hit
GGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG1060.14686525805334258No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC1040.14409421544856252No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT1040.14409421544856252No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA1040.14409421544856252No Hit
GTACTATACTTCACAACAATCCTAATCCTAATACCAACTATCTCCCTAAT1040.14409421544856252No Hit
CCATTAACCTTCCCTCTACACTTATCATCTTCACAATTCTAATTCTACTG1010.13993765154139245No Hit
CATATAACCTCCCCCAAAATTCAGAATAATAACACACCCGACCACACCGC1010.13993765154139245No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT1000.13855213023900242No Hit
GTACATGGGCGTCATATCGAAAGATATG1000.13855213023900242No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA1000.13855213023900242No Hit
CTTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATACCCCCGATTCCG1000.13855213023900242No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC990.1371666089366124No Hit
ACACAATGGGGCTCACTCACCCACCACATTAACAACATAAAACCCTCATT980.13578108763422236No Hit
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC960.13301004502944233No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA960.13301004502944233No Hit
GCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCA960.13301004502944233No Hit
CTATATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATACAT950.1316245237270523No Hit
AGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACG950.1316245237270523No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA950.1316245237270523No Hit
ATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCA940.13023900242466227No Hit
CCTTAACCTCTACTTCTACCTACGCCTAATCTACTCCACCTCAATCACAC930.12885348112227224No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAG890.12331139591271216No Hit
CCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACT890.12331139591271216No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA870.1205403533079321No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT860.1191548320055421No Hit
GCTATTACCTTCTTATTATTTGATCTAGAAATTGCCCTCCTTTTACCCCT850.11776931070315207No Hit
TAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAG840.11638378940076204No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC840.11638378940076204No Hit
TCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGT830.114998268098372No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC820.11361274679598198No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT810.11222722549359197No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA780.10807066158642188No Hit
AACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC770.10668514028403185No Hit
TTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTC770.10668514028403185No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA760.10529961898164185No Hit
GAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAAC760.10529961898164185No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA760.10529961898164185No Hit
GGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTG760.10529961898164185No Hit
CTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCAC750.10391409767925182No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG740.10252857637686179No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)