FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008171349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008171349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24750
Total Bases3.7 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGCGTCATATCGAAAGATATGACG2190.8848484848484849No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2040.8242424242424242No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1760.7111111111111111No Hit
GTACATGGGCGTCATATCGAAAGATATG1350.5454545454545455No Hit
CTTAGCAACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCAGGCTTAG1340.5414141414141413Clontech Universal Primer Mix Long (96% over 25bp)
ACTAGCAACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCAGGCTTAG1150.4646464646464647Clontech Universal Primer Mix Long (96% over 25bp)
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC970.39191919191919194No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG870.3515151515151515No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG840.3393939393939394No Hit
GTATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG820.3313131313131313No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA790.3191919191919192No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA770.3111111111111111No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG750.30303030303030304No Hit
ATTAGCAACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCAGGCTTAG690.2787878787878788Clontech Universal Primer Mix Long (96% over 25bp)
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG690.2787878787878788No Hit
GGTATCAACGCAGAGTACATGGGGG660.26666666666666666No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT610.24646464646464644No Hit
CCTTAGCAACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCAGGCTTA580.23434343434343435Clontech Universal Primer Mix Long (96% over 25bp)
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA570.2303030303030303No Hit
GTATCAACGCAGAGTACATGGGGGG520.2101010101010101No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA500.20202020202020202No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG480.19393939393939394No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA480.19393939393939394No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT470.1898989898989899No Hit
ACGCAGAGTACATGGGCGTCATATCGAAAGATATG450.18181818181818182No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC440.17777777777777778No Hit
GTATAAGGATGCTCTGTGAACTGTCATCGGTCCGATCAATTAGTCTAGTG430.17373737373737375No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA420.1696969696969697No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG420.1696969696969697No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC420.1696969696969697No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC390.1575757575757576No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT370.1494949494949495No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT370.1494949494949495No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG370.1494949494949495No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT360.14545454545454545No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT360.14545454545454545No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG340.13737373737373737No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG340.13737373737373737No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT340.13737373737373737No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG330.13333333333333333No Hit
CTTTAGGGTCTTGTAATTGGAATGAGTACAATTTAAATCCCTTAACGAGG320.1292929292929293No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC320.1292929292929293No Hit
TATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATG310.12525252525252525No Hit
ATCAACGCAGAGTACATGGGCGTCATATCGAAAGATATGACG310.12525252525252525No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG300.12121212121212122No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC300.12121212121212122No Hit
GCTTGAAGCAGATGATATCCCGATGCTGACGGCTTACAATAAACGTGATC290.11717171717171718No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG290.11717171717171718No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT290.11717171717171718No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT280.11313131313131312No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT270.1090909090909091No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC270.1090909090909091No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA270.1090909090909091No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG270.1090909090909091No Hit
GCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGT260.10505050505050505No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG260.10505050505050505No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC260.10505050505050505No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC250.10101010101010101No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)