FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008171634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008171634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18803
Total Bases3 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2191.1647077594000956No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1660.8828378450247301No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA1200.6381960325479976No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG1160.6169228314630644No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT860.457373823326065No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA820.4361006222411317No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC790.42014572142743173No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA770.40950912088496516No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG750.39887252034249854No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC700.3722810189863319No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT680.36164441844386536No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG650.3456895176301654No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT620.32973461681646543No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT590.3137797160027655No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG530.2818699143753656No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG530.2818699143753656No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG520.2765516141041323No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT520.2765516141041323No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA520.2765516141041323No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT500.2659150135616657No Hit
GCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCG490.2605967132904324No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA480.25527841301919907No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA460.24464181247673242No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG460.24464181247673242No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC460.24464181247673242No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG450.2393235122054991No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT430.2286869116630325No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT420.22336861139179917No Hit
GGTATCAACGCAGAGTACATGGGGG410.21805031112056586No Hit
GCTTGAAGCAGATGATATCCCGATGCTGACGGCTTACAATAAACGTGATC390.20741371057809924No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT380.20209541030686592No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG370.1967771100356326No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC370.1967771100356326No Hit
CATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCGGCAAT360.1914588097643993No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC360.1914588097643993No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC340.18082220922193268No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG340.18082220922193268No Hit
GTACTGGTAGGCGTTAGACCCCGTGACTCGTGGCTGAACGGCCGTACAAC330.17550390895069934No Hit
GCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGT320.17018560867946605No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA320.17018560867946605No Hit
TGATTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCC320.17018560867946605No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC320.17018560867946605No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA320.17018560867946605No Hit
GTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCCCGATGCTGAC310.16486730840823272No Hit
CTTTCACCCACAGCACCAATCCTACCTCCATCGCTAACCCCACTAAAACA310.16486730840823272No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT310.16486730840823272No Hit
GTATCAACGCAGAGTACATGGGGCTTGCT310.16486730840823272No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC310.16486730840823272No Hit
ACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGG300.1595490081369994No Hit
GATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGAC300.1595490081369994No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC290.1542307078657661No Hit
ATTAAAGAGTTAGCACCCACCATTGGATGAAGCCCAGGATAAGTGACCCC290.1542307078657661No Hit
GAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTT280.14891240759453278No Hit
GTCCATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTT280.14891240759453278No Hit
CCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCC280.14891240759453278No Hit
GTATCAACGCAGAGTACATGGGGAG270.14359410732329947No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT270.14359410732329947No Hit
CTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCT270.14359410732329947No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC270.14359410732329947No Hit
GATGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCC270.14359410732329947No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC270.14359410732329947No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT260.13827580705206616No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA260.13827580705206616No Hit
GTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAA260.13827580705206616No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT250.13295750678083285No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG250.13295750678083285No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT250.13295750678083285No Hit
ATACATAAGTGTATAATGTCTCGGACCCGTCGCAACGGTTGTTAATATGA250.13295750678083285No Hit
AGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTA240.12763920650959953No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT240.12763920650959953No Hit
ACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTG230.12232090623836621No Hit
GTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGC230.12232090623836621No Hit
GTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGA230.12232090623836621No Hit
GTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCT230.12232090623836621No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT220.11700260596713291No Hit
GTATCAACGCAGAGTACATGGGGGG220.11700260596713291No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC220.11700260596713291No Hit
GGTTAATAAGGCCTAGCCTAAAGGTTCTTGCAGAGCAACATCATATACCC220.11700260596713291No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG220.11700260596713291No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA220.11700260596713291No Hit
GTCAAAACCGCGCAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTG220.11700260596713291No Hit
TCATTGGAGTGCCAGTAATTCTACCACGCCTTATATTTAAAGACAAAAAG220.11700260596713291No Hit
GCTCCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAG220.11700260596713291No Hit
GGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGCTTATGA210.11168430569589959No Hit
GGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATT210.11168430569589959No Hit
TATCAACGCAGAGTACATGGGGCTTGCT210.11168430569589959No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC210.11168430569589959No Hit
CTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCG210.11168430569589959No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG210.11168430569589959No Hit
GCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTT210.11168430569589959No Hit
GCGTTAGACCCCGTGACTCGTGGCTGAACGGCCGTACAACTCGACAGCCG210.11168430569589959No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA210.11168430569589959No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT210.11168430569589959No Hit
CAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTT210.11168430569589959No Hit
GGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCCCTGCATCCA200.10636600542466629No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC200.10636600542466629No Hit
GATCATGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCAT200.10636600542466629No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT190.10104770515343296No Hit
CTATTAAAGAGTTAGCACCCACCATTGGATGAAGCCCAGGATAAGTGACC190.10104770515343296No Hit
CCCCGAGGAGCGGACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGG190.10104770515343296No Hit
TCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGG190.10104770515343296No Hit
GGCGCATAGTCGCCATTCACAGATTTGCTCGGCAATCAGTACTGGTAGGC190.10104770515343296No Hit
GTGACAAGGTTTCCCCCTAATCGAGACGCTGCAATAACACAGGGGCATAC190.10104770515343296No Hit
GCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTT190.10104770515343296No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT190.10104770515343296No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG190.10104770515343296No Hit
GGTATCAACGCAGAGTACATGGGGGG190.10104770515343296No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC190.10104770515343296No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)