FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008171706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008171706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108606
Total Bases18.6 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG6760.6224333830543433No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG6620.60954275086091No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC5000.46037972119404086No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC4870.44840984844299575No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC4090.37659061193672544No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA4020.3701452958400088No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC3950.36369997974329227No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA3920.360937701416128No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC3740.34436403145314254No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC3660.3369979559140379No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC3140.28911846490985765No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA3070.28267314881314104No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA2840.2614956816382152No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT2780.2559711249838867No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC2730.2513673277719463No Hit
GCATAATATTTCGCCCACTAAGCCAATCACTTTATTGACTCCTAGCCGCA2670.24584277111761782No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA2640.24308049279045357No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA2620.24123897390567742No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA2570.236635176693737No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG2440.2246653039426919No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT2390.22006150673075153No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA2380.21914074728836344No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC2330.21453695007642304No Hit
CCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGA2280.2099331528644826No Hit
CCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA2210.20348783676776605No Hit
GCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAA2200.202567077325378No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT2160.19888403955582562No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT2160.19888403955582562No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG2090.19243872345910906No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC2050.18875568568955675No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA2020.1859934073623925No Hit
CCCTATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATA2020.1859934073623925No Hit
CGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACC1990.18323112903522826No Hit
CCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCG1960.180468850708064No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG1950.17954809126567592No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT1910.1758650534961236No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA1900.17494429405373552No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1870.17218201572657127No Hit
GGTGTAGCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAA1820.16757821851463087No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT1770.16297442130269046No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA1760.16205366186030237No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT1740.16021214297552622No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG1690.15560834576358581No Hit
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC1660.15284606743642157No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT1660.15284606743642157No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA1610.14824227022448117No Hit
CTATATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATACAT1600.14732151078209307No Hit
ATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCA1580.14547999189731692No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTG1570.14455923245492883No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA1560.14363847301254073No Hit
GTACTATACTTCACAACAATCCTAATCCTAATACCAACTATCTCCCTAAT1510.13903467580060033No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC1490.13719315691582418No Hit
CCATATATCCAAACAACAAAGCATAATATTTCGCCCACTAAGCCAATCAC1490.13719315691582418No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT1460.13443087858865993No Hit
TAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCA1460.13443087858865993No Hit
CGTACACACCGCCCGTCACCCTCCTCAAGTATACTTCAAAGGACATTTAA1450.13351011914627184No Hit
GTGTAGCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAA1440.13258935970388377No Hit
ATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCA1420.1307478408191076No Hit
CTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGCATTACT1410.12982708137671953No Hit
GCCCATGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC1380.12706480304955528No Hit
GTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCTCCTCAAG1380.12706480304955528No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT1370.1261440436071672No Hit
CGTAAAGACGTTAGGTCAAGGTGTAGCCCATGAGGTGGCAAGAAATGGGC1370.1261440436071672No Hit
CCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGC1350.12430252472239102No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA1340.12338176528000294No Hit
ACTGAGAGTAGAGTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACC1330.12246100583761486No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA1330.12246100583761486No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGG1320.12154024639522679No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC1320.12154024639522679No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT1320.12154024639522679No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC1310.12061948695283871No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1310.12061948695283871No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT1290.11877796806806253No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA1270.11693644918328637No Hit
GACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGC1260.11601568974089829No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA1250.11509493029851021No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG1240.11417417085612214No Hit
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG1230.11325341141373406No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT1220.11233265197134595No Hit
GTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAAT1220.11233265197134595No Hit
GAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAAC1220.11233265197134595No Hit
CCATAAACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTC1210.11141189252895788No Hit
GTGCTTAGTTGAACAGGGCCCTGAAGCGCGTACACACCGCCCGTCACCCT1210.11141189252895788No Hit
GGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG1200.1104911330865698No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG1190.10957037364418172No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA1190.10957037364418172No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCA1190.10957037364418172No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG1190.10957037364418172No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG1180.10864961420179364No Hit
ACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGG1170.10772885475940557No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA1170.10772885475940557No Hit
CTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC1170.10772885475940557No Hit
ACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA1160.10680809531701749No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCC1160.10680809531701749No Hit
GCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAA1160.10680809531701749No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1160.10680809531701749No Hit
CTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCT1150.10588733587462938No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA1150.10588733587462938No Hit
CCCCCCACCCTACCACACATTCGAAGAACCCGTATACATAAAATCTAGAC1150.10588733587462938No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT1140.1049665764322413No Hit
GCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGCC1130.10404581698985323No Hit
CCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCTACTTCTAACC1130.10404581698985323No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT1120.10312505754746515No Hit
ACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACC1110.10220429810507707No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT1100.101283538662689No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)