FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008172744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008172744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13304082
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCTATA3303052.482734246526743No Hit
GGCGATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCTATA2766772.0796399180341796No Hit
CGGGATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCTATA1289120.9689657655447403No Hit
GGGACCGCGACGCGGCGCGTGCGCGGGCGGGGCCCGCGTGCCCGTTCGTTC1243340.934555274088058No Hit
GGCATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCTATAC905680.6807534710023586No Hit
GGGGAAAGATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCC873430.6565127905856263No Hit
GGGAGATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCTAT743880.5591366619658538No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG686500.5160070420491997No Hit
GGGATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGA612840.460640576328378No Hit
GGGATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCTATAC597900.4494109401911383No Hit
GGCAGATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCTAT584200.43911334882030945No Hit
GGCCCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCC584100.43903818391979244No Hit
GCCGATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCTATA559370.42044990402193855No Hit
CGCGATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCTATA553600.4161128892621077No Hit
GGGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAA499530.37547122755256623No Hit
GGCAAGATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCTA476980.3585215424859829No Hit
GCGGATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCTATA467070.3510727008447483No Hit
GGGATAGCCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATA436270.3279219114855125No Hit
CCGGATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCTATA417870.31409156979038466No Hit
GGCGAAAGATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCC410220.3083414549008342No Hit
GGCCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGAC407550.3063345520570303No Hit
GGGATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCC402050.3022004825285954No Hit
GGGAGCCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAAT378760.28469457719818625No Hit
GGGAAGATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCTA369870.278012417542225No Hit
GGGAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAA369230.2775313621789162No Hit
GGGAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAA367080.27591531681780074No Hit
GGGAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAAT339720.25535020003634973No Hit
GGCCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCG339150.25492176010340284No Hit
CGGGAAAGATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCC326010.24504509217546916No Hit
CGGAGATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCTAT310830.23363506027698866No Hit
GGGATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAA304310.22873430876328033No Hit
GGGAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGA302260.22719342830268183No Hit
GGGGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGC298870.22464533817515556No Hit
GGGGACGCGGCGCGTGCGCGGGCGGGGCCCGCGTGCCCGTTCGTTCGCTCG289990.21797069500924604No Hit
GGGGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAA279680.21022119376594342No Hit
GGCCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAA271140.20380211126179168No Hit
GGCCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATATAGCAAGGAC262150.19704478670531345No Hit
GGGCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATATAGCAAGGAC259640.19515814770233675No Hit
GGGAAAGATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCT246580.18534161169481667No Hit
GGCAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAAT242470.18225233428356802No Hit
GGCAAAGATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCT241280.18135787196741573No Hit
GGGAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTT239640.18012516759893693No Hit
GGGGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGG236400.17768982482218615No Hit
GGGTAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCC231950.1743449867491797No Hit
CGGATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCTATAC228710.17190964397242892No Hit
CGGATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGA226520.17026353265110664No Hit
GGCACCGCGACGCGGCGCGTGCGCGGGCGGGGCCCGCGTGCCCGTTCGTTC217400.1634084937239563No Hit
GGGACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAAC216920.1630477022014747No Hit
GGGCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCT215260.16179996485289252No Hit
CGGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAA213910.16078523869591302No Hit
GGCAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGC211390.15889108320288464No Hit
GGCAGCCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAAT210570.15827473101864525No Hit
GGGAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGG208490.15671130108789166No Hit
GGGGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAG201840.15171283520351123No Hit
GGGGGGTTGGGACGCGGCGGCCGGCGGGCGGTGGGTGTGCGCGCCCGGCGC200270.1505327462653943No Hit
CGGACCGCGACGCGGCGCGTGCGCGGGCGGGGCCCGCGTGCCCGTTCGTTC196010.14733072150337018No Hit
GGGAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAA183350.13781484509791808No Hit
GGGACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAG178270.1339964681516545No Hit
GGGAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCA171920.12922349696882507No Hit
GGGGAGTCGGGGGAGGATCCCGCCGGGCCGGGCCCGGCGTCCCAGCGGGTT171310.12876499107567135No Hit
GGCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCT170900.12845681498355166No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTG168830.12690090154284978No Hit
GGGGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCTATACCT156330.11750528897822488No Hit
GGGGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCC156160.11737750864734599No Hit
GGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAA153310.11523530898261149No Hit
GGCCCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGG152110.11433333017640751No Hit
GGGGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAA150090.11281499918596412No Hit
GGGAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGA149490.11236400978286212No Hit
GGGATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA147020.11050743674009225No Hit
GGGAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAG145110.10907178714021756No Hit
CGGAAGATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCTA142660.10723024707755108No Hit
CCCGATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCTATA142170.10686193906501779No Hit
GGGATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAA141380.1062681363509335No Hit
GGGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCC140920.1059223778085553No Hit
GGGGCGACGCGGCGCGTGCGCGGGCGGGGCCCGCGTGCCCGTTCGTTCGCT139030.10450176118878401No Hit
GGGGAGTCGGGGGAGGATCCCGCCGGGCCGGGCCCGGCGTTCCCAGCGGGT138380.10401318933542351No Hit
GGCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAA137520.10336677119097731No Hit
GGGGGGAAAGATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACC135770.10205138543192985No Hit
GGGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGC134420.10103665927495036No Hit
CGCATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCTATAC134050.10075854914303746No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)