FastQCFastQC Report
Thu 9 May 2024
EGAF00008175745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008175745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50122002
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length10-85
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGGCCTGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1843160.3677347125918873Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGATGGCCTGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCA752330.1500997506045349TruSeq Adapter, Index 5 (97% over 36bp)
GGATGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAA721970.1440425304639667TruSeq Adapter, Index 6 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)