FastQCFastQC Report
Sat 11 May 2024
EGAF00008176053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008176053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48208324
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length10-85
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAAGATAGTTACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA8947021.8559077058974298Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATAAGATAGTTACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6161051.2780054332525645Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATAAGATAGTTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT2929420.6076585446115074TruSeq Adapter, Index 8 (100% over 36bp)
GATAAGATAGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC2885790.5986082403528487TruSeq Adapter, Index 5 (100% over 35bp)
TAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCTCGAA1410600.2926050696141189TruSeq Adapter, Index 13 (97% over 37bp)
TAGAACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCTCGA1387150.2877407644372785TruSeq Adapter, Index 13 (97% over 37bp)
GATAAGATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCTC1063800.22066728559159204TruSeq Adapter, Index 13 (97% over 37bp)
GATAAGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCTCG1002610.20797445685935897TruSeq Adapter, Index 13 (97% over 37bp)
GATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCTCGAATCT912380.18925777216399392TruSeq Adapter, Index 13 (97% over 37bp)
GATAAGATAGTTACCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA840840.17441801129614046Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GATAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCTCGAA688850.1428902610262908TruSeq Adapter, Index 13 (97% over 37bp)
GATAAGATAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTC668970.138766491861447TruSeq Adapter, Index 13 (97% over 37bp)
GATAAGATAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCT668460.13866070100259034TruSeq Adapter, Index 13 (97% over 37bp)
GATAAGATAGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTC665090.13796165160191007TruSeq Adapter, Index 13 (97% over 37bp)
GATAAGATAGTTACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA652860.1354247453199161Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCTCGAAT559590.11607746413254275TruSeq Adapter, Index 13 (97% over 37bp)
GATAAGATAGTTATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA536230.1112318279307947Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATAAGATAGTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC513520.10652102321582473Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)