FastQCFastQC Report
Thu 31 Aug 2023
EGAF00008178841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008178841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15745477
Sequences flagged as poor quality0
Sequence length58
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA471570.29949553131988316Clontech SMARTer II A Oligonucleotide (100% over 25bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN290480.18448472535954294No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG238310.15135140078639725No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGATCGGAAGAGCGTCGTGTAGGGAAAG193560.12293054062445996Illumina Single End PCR Primer 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)